摘要
目的探讨转化生长因子β1(TGF-β1)、血小板源性生长因子(PDGF)、结缔组织生长因子(CTGF)在尘肺病发生、发展中表达水平的变化及其意义。方法采用双抗体夹心法,测定70例尘肺病患者(其中包括29例矽肺病患者和41例煤工尘肺病患者)和77例健康对照者血清TGF-β1、PDGF、CTGF的表达水平。结果尘肺病患者血清TGF-β1、PDGF、CTGF含量分别为(44.95±23.72)ng/mL、(56.95±55.68)ng/mL、(346.70±259.49)pg/mL,对照组则分别为(6.81±4.99)ng/mL、(30.96±21.63)ng/mL、(307.49±235.40)pg/mL,病例组与对照组血清TGF-β1、PDGF含量差异均有统计学意义(P<0.05),血清CTGF含量在两组间差异无统计学意义(P>0.05);矽肺病患者血清TGF-β1、PDGF含量均高于煤工尘肺病患者,差异有统计学意义(P<0.05);尘肺病患者血清TGF-β1、PDGF含量随着期别的增加而降低,差异有统计学意义(P<0.05);两变量相关分析显示TGF-β1与PDGF、CTGF与PDGF呈正相关关系(P<0.05)。结论血清TGF-β1、PDGF表达水平与尘肺病的发生发展、病理类型及严重程度密切相关。
Objective To explore the TGF-β1, PDGF, CTGF serum expression significance in the occurrence and development of pneumoconiosis. Methods The serum levels of TGF-β1, PDGF, CTGF in 70 patients with pneumoconiosis （including 29 patients with silicosis and 41 patients with coal pneumoconiosis） and 77 healthy individuals were detected by means of enzyme linked immunosorbent assay （ELISA）. Results The serum levels of TGF-β1, PDGF, CTGF in patients with pneumoconiosis were（44. 95±23.72） ng/mL, （56.95±55.68） ng/mL, （346. 70±259.49） pg/mL, the serum levels of control group were （6. 81±4. 99） ng/mL, （30. 96±21.63） ng/mL, （307. 49±235.40） pg/mL. There were significantly statistical differences between the case group and the control group in the serum levels of TGF-β1 and PDGF（P〈0. 05）. There was no significantly statistical difference between the two groups in the serum levels of CTGF（P〉0.05）. The serum levels of TGF-β1 and PDGF in the patients with silicosis were higher than those in the patients with coal pneumoconiosis, and there was significantly statistical difference between the two groups（P〈0.05）. The serum levels of TGF-β1 and PDGF in the patients with pneumoconiosis decreased with the pneumoconiosis stage going up （P〈0. 05）. There was the bivariate correlation not only between the serum levels of TGF-β1 and PDGF in the whole objects but also the serum levels of CTGF and PDGF（P〈0.05）. Conclusions The serum levels of TGF-β1, PDGF, CTGF in the patients with pneumoconiosis may correlate with the pathological stages, styles and degree of pneumoconiosis.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期754-756,793,共4页
Journal of Sichuan University(Medical Sciences)
