摘要
目的应用蛋白质组学技术,建立晕船适应及不适应大鼠脑干蛋白质双向电泳图谱,鉴定差异表达蛋白。方法应用双向电泳技术,对2组40只大鼠脑干蛋白进行分离,肽指纹图谱鉴定差异蛋白质,并观察谷氨酰胺合成酶活力变化。结果获得8个晕船适应相关蛋白质,其中硫氧还蛋白过氧化物酶Ⅱ、低相对分子质量神经丝蛋白、泛素羧基末端水解酶PGP9.5和谷氨酰胺合成酶上调;碳酸酐酶Ⅱ、磷酸丙糖异构酶Ⅰ、磷酸甘油酸变位酶B和线粒体电压依赖型阴离子通道下调。谷氨酰胺合成酶在晕船适应组大鼠活力增强。结论大鼠对晕船适应后可诱导与能量代谢、神经递质和氧化应激相关的脑干蛋白质组的改变。
Objective To establish a two-dimensional eletrophoresis system for brainstem proteins between seasickness adaptive and unadaptive rats by using proteomics technology and to identify differentially expressed proteins. Methods The brainstem proteins of two groups were isolated by two-dimensional electrophoresis, respectively. The differentially expressed proteins were identified by peptide mass fingerprint （PMF） and the changes of glutamine synthetase activities were investigated. Results 8 seasickness adaptive proteins were identified by PMF. Peroxiredoxin Ⅱ ,light molecular-weight neurofilament, ubiquitin carboxyl-terminal hydrolase PGP9.5 and glutamine synthetase were up-regulated, while carbonic anhydrase Ⅱ, triosephosphate isomerase Ⅰ , phosphoglycerate mutase isozyme B and mitochondrial voltage dependent anion channel were down-regulated. Moreover, the glutamine synthetase activities of seasickness adaptive rats were much higher than those of the unadaptive ones. Conclusion Changes of brainstem protein expressions in seasickness adaptive rats could be associated with energy metabolism, neurotransmitter adjustment and oxidative stress.
出处
《中国职业医学》
CAS
北大核心
2006年第4期244-246,共3页
China Occupational Medicine
基金
军事医学专项基金(2001J-4)
关键词
晕船
蛋白质组学技术
蛋白表达
大鼠
Seasickness
Proteomics technology
Protein expression
Rat
