摘要
目的建立愈伤灵胶囊中人参皂苷Rg1的含量测定方法。方法采用高效液相色谱法。色谱柱为HypersilBDSC18柱(150mm×4.6mm,5μm);流动相:乙腈-0.5%磷酸溶液(24∶76);流速:1.0mL/min;柱温:25℃;检测波长:205nm。结果人参皂苷Rg1进样量在0.05～0.80μg内呈现良好的线性关系,回归方程为:Y=3629375.5X+2517.1,r=0.9998,平均回收率为100.48%,RSD=1.59%(n=5)。结论测定方法简便、准确、重现性好,适用于愈伤灵胶囊中人参皂苷Rg1的含量测定。
Objective To established a method for the content determination of ginsenoside Rg1 in Yushangling Capsules by HPLC. Methods HPLC was used with the C18 column. The mobile phase was acetonitrile-0.5% phosphoric acid （24 ： 76）. The flow rate was 1.0 mL/min, the column temperature was 25℃, and the detection wave was set at 205 nm. Results The calibration curve was linear in the range of 0.05-0.80μg. The regression equation was Y=3629375.5X＋2517.1, r=0.9998. The average recovery was 100.48% and RSD was 1.59%. Conclusion The method is simple, accurate and suitable for the content determination of ginsenoside Rg1 in Yushangling Capsules.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第8期44-45,共2页
Chinese Journal of Information on Traditional Chinese Medicine
