摘要
目的采用HPLC法测定山楂精降脂片中熊果酸的含量。方法色谱柱为KromasilC18(4.6mm×200mm,5μm),流动相为甲醇-0.15%磷酸溶液(88∶12),流速1.0mL/min,检测波长215nm,柱温为室温。结果熊果酸进样量在0.253～5.052μg范围内与峰面积呈良好的线性关系,相关系数r=0.9998(n=6),平均回收率为100.37%,RSD=1.88%。结论本方法操作简便,分离效果较好,灵敏度高,重现性好,结果准确,可较好控制山楂精降脂片的质量。
Objective To determine ursolic acid in Shanzhajing Jiangzhi Tablets by HPLC method. Me,eel Chromatographic column was Kromasil C18 （4.6 mm×200 mm, 5μm）, The Mobile phase was methanol- 0.15% phosphoric acid （88 ： 12）, and the flow rate was 1.0 mL/min. Detection wavelength was 215 nm. Column temperature was room temperature. Results Ursolic acid quantity and its peak area showed good linearity relationship in the range of 0.253-5.052μg. The correlation coefficient （r） was 0.9998 （n =6）. The average recovery was 100.37% and RSD was 1.88%. Conclusion The method is easy and accurate, with good sensitivity and repeatability. It is a good method to control the quality of Shanzhajing Jiangzhi Tablets.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第8期41-43,共3页
Chinese Journal of Information on Traditional Chinese Medicine
