摘要
乙醛在黄嘌呤氧化酶催化作用下，被空气氧化生成乙酸和过氧化氢，酶反应生成的过氧化氢氧化新吖啶酯试剂［１０－甲基－９－（对甲酰苯基）吖啶羧酸酯氟磺酸盐］产生化学发光，化学发光强度与己醛浓度在一定范围内成线性关系．由此建立了一种化学发光分析新体系，用于测定乙醇含量．线性范围２．０×１０－７～１．２×１０－３ｍｏｌ／Ｌ，测定５．０×１０－７ｍｏｌ／Ｌ乙醛，相对标准偏差为６．８％，检测限为１．２×１０－７ｍｏｌ／Ｌ，测定环境水样中乙醇含量，取得满意结果，回收率为９５～１０６％．
A new chemiluminescence analytical system of xanthine oxidase-acetaldehyde-Acridinium was studied. This system is based on the quantitative oxidation of acetaldehyde by xanthine oxidase to form acetic acid and hydrogen peroxide. The hydrogen peroxide produced is then measured by a new acridinium ester [10-methyl-9-(p-formylphenyl)acridinium carboxylate fluorosulphonate]CL system. In addition, the effect of other substances on the test was investigated. The appropriate conditions for the ezymatic reaction and the chemiluminescent reaction were studied. In addition, the effect of other substances on the acetaldehyde determination was also investigated. The graph was linear over three orders of magnitude between 2. 6×10-7 and 1. 2×10-3mol/L and the relative standard deviation (n=10) was 6. 8% for a 5. 0×10-7mol/L acetaldehyde. The detection limit for acetaldehyde by this method was about one order of magnitude better than those of other chemiluminescence methods; The determination of acetaldehyde in certain polluted water, the results were satisfactory.
出处
《发光学报》
EI
CAS
CSCD
北大核心
1996年第3期245-250,共6页
Chinese Journal of Luminescence
基金
国家自然科学基金
关键词
黄嘌呤氧化酶
乙醛
化学发光分析
xanthine oxidase, acetaldehyde, chemiluminescence analysis, 10-Methyl-9(p-formylphenyl)-acridinium carbxylate fluorosulphonate
