摘要
目的研究氟哌利多复合丙泊酚对大鼠海马锥体细胞钠通道电流的影响。方法酶消化法急性分离SD大鼠（10～14d）海马锥体细胞，全细胞膜片钳技术记录氟哌利多复合丙泊酚对钠通道电流的影响。结果在钳制电压（Vn）-80mV，刺激电压（V1）0mV条件下，氟哌利多单独使用对钠电流抑制作用的半数抑制浓度（IC50）为（26．01±5．08）μmol／L（n=7），丙泊酚单独应用时对钠电流抑制作用的IC50为（44．53±3．89）μmol／L（n=6）。1／4和3／4 IC50的氟哌利多与丙泊酚合用，对钠电流抑制作用的IC50值分别为（32．34±9．56）μmol／L（n=6）和（30．73±5．77）μmol／L（n=6）。等效线图分析结果显示1／4 IC50的氟哌利多与丙泊酚合用时表现为相加作用；3／4 IC50的氟哌利多与丙泊酚合用时表现为拮抗作用。结论氟哌利多和丙泊酚对海马锥体细胞钠通道电流均有明显的抑制作用，并呈浓度依赖性；较低浓度的氟哌利多与丙泊酚复合时，对钠通道电流的抑制作用表现为相加作用；而较高浓度的氟哌利多与丙泊酚复合时，表现为拮抗作用。
Objective To study the effects of droperidol combined with propofol on the wholecell sodium channel in rat hippocampal paramidal neurons. Methods Whole-cell patch-clamp recordings were made from enzymatically isolated rat hippocampal paramidal neurons. Results Both of droperidol and propofol dose-dependently blocked the whole-cell sodium currents evoked by a voltage step to 0 mV from a holding potential of -80 mV with a mean IC50 values of （26.01±5.08） μmol/L （n=7） and （44. 53±3.89） μmol/L（n=6）. When propofol was administered combined with 1/4 IC50 droperidol, the IC50 value for blocking sodium current was （32.34 ±9.56） μmol/L （n = 6）, which located in the 95 % confidence limits of theoretical additive line. When propofol was administered combined with 3/4 IC50 droperidol, the IC50 value for blocking sodium current was （30.73±5.77） μmol/ L（n=6）, which located out of the 95% confidence limits of theoretical addictive line. Conclusion Both of droperidol and propofol have a significant inhibition on sodium current of hippocampal paramidal neurons in a dose-dependent manner. Lower dose droperidol combined with propofol additively potentiates the block of hippocampal sodium current. Higher dose droperidol combined with propofol antagnizes the block of hippocampal sodium current.
出处
《临床麻醉学杂志》
CAS
CSCD
2006年第7期522-524,共3页
Journal of Clinical Anesthesiology
关键词
氟哌利多
丙泊酚
海马
钠通道
Droperidol
Propofol
Sodium Channel
Hippocampal
