摘要
目的观察银杏叶提取物对急性缺血-再灌注损伤小鼠肾脏的保护作用。方法取昆明种小鼠30只,随机分为假手术组、模型组和给药组各10只。给药组小鼠腹腔注射银杏叶提取物,0.1 mg.g-1;模型组与假手术组均腹腔注射等量0.9%氯化钠注射液。30 m in后将所有小鼠麻醉,切除给药组与模型组小鼠右侧肾脏,游离左侧肾脏,夹闭左侧肾蒂45 m in,然后去除血管夹,再灌注24 h;将假手术组小鼠麻醉后只切除右肾,缝合切口。24 h后处死小鼠,测定血清肌酐(Cr)和尿素氮(BUN)并观察肾脏组织的病理学改变。结果模型组小鼠血清Cr[(49.90±12.02)μmol.L-1]与BUN[(26.36±7.41)mmol.L-1]均明显高于假手术组[分别为(21.40±2.67)μmol.L-1和(7.20±0.84)mmol.L-1](均P<0.01);给药组小鼠血清Cr[(30.20±6.30)μmol.L-1]与BUN[(15.37±3.77)mmol.L-1]均较模型组明显降低(均P<0.01),与假手术组相比,均差异无显著性。模型组小鼠肾小管上皮细胞缺血性改变明显,给药组小鼠肾小管上皮细胞缺血性改变较模型组明显减轻。结论银杏叶提取物对急性肾缺血-再灌注损伤小鼠肾脏有明显的保护作用。
Objective To study the protective effects of Ginkgo bilobae extract（EGb） on the mouse with a model of acute ischemia-reperfusion injury of the kidney. Methods 30 mice of the Kunning strair were randomly divided into 3 equal groups ： the sham operation group, ischemia-reperfusion model group, and trial group. Mice of the trial group were given each an intraperitoneal injection of 0.1 mg·g^-1 of EGb, while those of the model group and sham operation group were given each an intraperitoneal injection of equivalent amount of 0.9% sodium chloride solution. 30 min later, mice of the 3 groups were subjected to chloral hydrate anesthesia. The right kidney of each of the mice of the trial group and model group was removed. The left kidney was then exposed an.d the renal pedicle was clamped for 45 min followed by declamping and reperfusion for 24 h. Mice of the sham operation group were submitted only to right side nephrectomy followed by suturing of the surgical incision. 24 h after the operation, mice of all 3 groups were sacrificed. Serum creatinine（Cr） and blood urea nitrogen（ BUN ） were determined and the left kidney was subjected to histopathological examination. Results The serum Cr[ （49.90 ± 12.02） μmol ·L^-1 ] and BUN [ （26.36±7.41 ） mmol ·L^-1 ] in mice of the model group were significantly higher than those [ （ 21.40 ±2.67 ）μmol ·L^-1 ] and [ （7.20±0.84） mmol ·L^-1 ] in mice of the sham operation group, respectively（P〈0.01, P〈0.01 ）. In contrast, the serum Cr[ （30.20±6.30）μmol ·L^-1] and BUN[ （ 15.37 ±3.77） mmol ·L^-1 ] in mice of the trial group were significantly lower than those in mice of the model group, respectively（P〈0.01, P〈0.01 ）. Although these values were higher in mice of the trial group than those in mice of the sham operation group, the differences, however, were statistically insignificant. Striking ischemic changes in the renal tuberlar epithelial cells in mice of the model group were demonstrated while these changes were trivial in the renal tubular epithelial cells in mice of the trial group. Conclusion EG1 was shown to exert dramatic protective effects on the mouse with a model of acute ischemia-reperfusion injury of the kidney.
出处
《医药导报》
CAS
2006年第8期737-739,共3页
Herald of Medicine
基金
深圳市科技计划项目(项目编号:2003-04029)
关键词
银杏叶提取物
缺血-再灌注损伤
肾脏
Ginkgo biloba extract （EGb）
Ischemia-reperfusion injury
Kidney
