摘要
目的建立快速检测TORCH-IgM抗体的方法。方法利用聚乙二醇(PEG)能加速抗原、抗体反应的特点,在常规间接EL ISA法检测TORCH-IgM抗体基础上,在样本稀释液、酶标记物稀释液中各加入3%PEG,以缩短反应时间。结果温育时间缩短到15 m in(37℃)的快速EL ISA法对检测人TORCH-IgM抗体强阳性、弱阳性、阴性标本具有稳定性好,精密性、特异性强的特点;稀释液中不加PEG的常规15 m in法,采用37℃、15 m in试验条件,可能会造成弱阳性标本漏检情况。结论快速EL ISA法的建立为TORCH病原体感染的快速检测和流行病学调查提供了新的手段。
[Objective] Establishing a fast method for detecting TORCH-IgM antibodies. [Methods] The PEG could accelerate the reaction between the antigen and the antibody. On the basement of the indirect ELISA method for detecting TORCH-IgM antibody, 3%PEG was added to the sample dilution liquid and the enzyme marker dilution liquid to shorten the reaction time. [Results] The new method was stable, accurate, and specific to detect strong positive, weak positive and negative TORCH-IgM antibodies strong positive, weak positive, and negative, using the new method, as the incubation time shortened to 15 min at 37℃. The weak positive samples might be failed to detect by the normal method 15′in dilution liquid without PEG when the incubation time shortened to 15 min at 37℃. [Conclusion] Fast ELISA provides a new method for fast detecting TORCH-IgM antibodies and for epidemiological study.
出处
《山东医药》
CAS
北大核心
2006年第20期1-3,共3页
Shandong Medical Journal
基金
山东省计划生育科学技术发展计划资助项目(鲁人口发[2005]70号)。
