摘要
目的:从“肿瘤异质化”角度出发,通过多种基因编码蛋白(Bcl-2／Bax,Cath-D和VEGF)在肠型胃癌和弥漫型胃癌中的表达,探讨二型胃癌不同生物学特性的分子学基础．方法:采用免疫组化SP法检测Bcl-2／Bax, cathepsin-D(Cath-D)和VEGF在胃癌及正常胃组织的表达．结果:Bcl-2／Bax,Cath-D和VEGF阳性表达率在胃癌组织中与癌旁正常胃黏膜组织中比较 (分别为44．59％ vs 27.30％;58．11％ vs 71．62％; 77．00％vs 21．60％;71．62％vs 14．86％)均有显著性差异(P<0．05),弥漫型胃癌中Bcl-2和 Cath-D阳性细胞指数明显高于肠型胃癌(分别为29．14±24．70 vs 19．80±27．37和52．59± 33．76 vs 29．21±39．67),而Bax阳性细胞指数 (分别为32．56±35．55 vs 47．74±27．43)明显低于肠型胃癌,差异均有显著性(P<0．05),VEGF 阳性细胞表达指数在不同胃癌浸润深度组间比较(早期胃癌7．13±15．81 vs 肌层浸润62．77 ±44．37 vs 浆膜浸润40．82±42．70)及有无淋巴结转移组间比较(42．05±42．61 vs 34．15± 42．52)有显著性差异(P<0．05)．结论:Bcl-2／Bax,Cath-D和VEGF分别参与了胃癌的发生,浸润进展和转移．二型胃癌在组织学发生相同的基础上由于在胃癌演进过程中有不同附加基因参与(Bcl-2／Bax和Cath-D), 使弥漫型胃癌获得了与肠型胃癌有所不同的亚克隆过程,因而表现为生物学特性的异质化．
AIM： To explore distinctive biological behavior in the intestinal-type gastric carcinoma and diffuse-type gastric carcinoma, according to the expression of Bcl-2, Bax, Cath-D and vascular epithelial growth factor （VEGF）.
METHODS： Immunohistochemistry SP method was performed to detect the expression of Bcl-2, Bax, Cath-D and VEGF in 36 cases of intestinal- type gastric carcinoma, 38 cases of diffuse-type gastric carcinoma, and their corresponding normal gastric tissues. RESULTS： The expression of Bcl-2, Bax, Cath-D and VEGF were significantly higher in gastric carcinoma than those in the normal controls （44.59% vs 27.30%; 58.11% vs 71.62%; 77.00% vs 21.60%; 71.62% vs 14.86%; all P〈0.05）. The posi-tive expression of Bcl-2 and Cath-D were markedly higher in diffuse-type gastric carcinoma than those in intestinal-type one （29.14±24.70 vs 19.80±27.37; 52.59±33.76 vs 29.21±39.67; both P〈0.05）, while the expression of Bax was notably lower in the former （32.56±35.55 vs 47.74±27.43, P〈0.05）. The positive rate of VEGF expression was closely correlated with the invasion depth （7.13±15.81, 62.77± 44.37, and 40.82±42.70 for mucosal or sub-mucosal invasion, muscular invasion, and serosa invasion, respectively） and lymph node metastasis （metastasis vs non-metastasis： 42.05±42.61 vs 34.15 ±42.52） of gastric carcinoma.
CONCLUSION： Bcl-2, Bax, Cath-D and VEGF are involved in the development and metastasis of gastric cancer. Due to the involvement of different genes, the intestinal-type or diffuse-type gastric carcinoma shows a distinctive biological behavior.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第16期1571-1577,共7页
World Chinese Journal of Digestology
