摘要
用自制的三系抗重组人α_(2a)型干扰素（rHu－IFN－α_(2a)）单克隆抗体，经中和试验，ELISA竞争试验和ELISA夹心试验，对α_(2a)型干扰素抗原表位与抗病毒作用之间的关系，以及干扰素激活靶细胞时与受体作用的方式等进行了研究，并提出了以下设想：（1）α_(2a)型干扰素分子只有一种抗病毒活性区。（2）活性区内至少有2个抗原表位。（3）干扰素抗病毒活性是其整个活性区与靶细胞上受体作用的结果，活性区内任何一个表位都不能单独完成抗病毒的作用。（4）活性区内每个表位对整个活性区的活性都非常重要，缺一不可。
The relationship between the antigenic epitopes of rHu-IFN-α 2a and its an-tiviral effect, as well as the mode of action of it on target cell receptor were studied by neu-tralization test, competitive ELISA and double McAb sandwich ELISA using the McAbs to rHu-IFN-α 2a prepared by the authors from 3 various cell strains. On the basis of the study,the following hypothesises was advanced: (1) IFN-α 2a molecule has only one antiviral active region; (2) There are at least 2 antigenic epitopes in the region; (3) The antiviral activity of IFN is the result of the action of whole active region on target cell receptors, and any single epitope in the region can not play antiviral role alone; (4) Each epitope in the region is very important for the antiviral activity, so any of them is indispensable.
出处
《中国生物制品学杂志》
CAS
CSCD
1996年第3期114-116,共3页
Chinese Journal of Biologicals
