摘要
从欧洲红豆杉（Ｔａｘｕｓｂａｃｃａｔａ）的嫩茎及什叶诱导出愈伤组织，并对之进行了愈伤组织培养及细胞悬浮培养研究；利用ＨＰＬＣ方法测定了培养物合成紫杉醇的能力．探索了提高培养细胞生长率及紫杉醇含量的一些措施．结果表明，欧洲红豆杉愈伤组织及悬浮培养细胞的生长速率已分别达到０．２７ｇ·Ｌ－１·ｄ－１和０．３５ｇ·Ｌ－１·ｄ－１，紫杉醇含量分别为０．００３１％和０．０１７％。
Calli were induced from new stems and needles of Taxus baccata,and the callus culture and cell suspension culture were studied. The taxol produced from the culture cells were analyzed by HPLC method. Some conditions able to increase the growth rate and taxol content of the culture cells were tested. The growth rates of calli and suspension culture cells from Taxus baccata were 0. 27 and 0. 35 g' L-1' d-1' (dry weight),and their taxol contents were 0. 0031 and 0. 017%, resmpively.
出处
《应用与环境生物学报》
CAS
CSCD
1996年第3期220-224,共5页
Chinese Journal of Applied and Environmental Biology
基金
中国科学院"八五"重点科研项目
关键词
红豆杉
欧洲红豆杉
细胞培养
Taxus baccata
taxol
callus culture
cell suspension culture
