摘要
目的探讨BALB／c小鼠系统感染白念珠菌后IL-18，IL-4，IFN-γ的动态变化。方法BALB／c小鼠随机分成4组：高菌量感染组，低菌量感染组，免疫功能低下组，正常对照组。分别于系统感染白念珠菌1，4，7天后应用ABC-ELISA检测各组小鼠的IL-18，IL-4，IFN-γ的水平。结果IL-18水平从感染第1天骤降后（但仍高于免疫功能低下组，P〈0．05～0．001），至感染后第7天达正常水平；而IFN-γ始终高于正常对照组及免疫功能低下组（P〈0．05）；IL-4于感染1天后低于正常对照组（但仍高于免疫功能低下组，P〈0．05～0．001），感染4天后则与正常对照组差异无显著性，7天后高于正常对照组（P〈0．05）。结论高低菌量白念珠菌感染的BLAB／c小鼠，免疫系统早期多以Th1型细胞应答为主；后期Th1／Th2型细胞因子均升高，表明细胞免疫和体液免疫均参与了抗白念珠菌感染。
Objective Exploring the kinetics of IL-18,IL-4,IFN-γ in BALB/c mice systemically infected with Candida Albicans. Methods BALB/c mice were divided into 4 groups randomly：the high dose infected group,the low dose infected group, the low immunologic function group, the normal control group. ABC-ELISA was used to detect these mice immunological function after infected CA（Candida Albicans） at the 1^st ,4^th ,7^th day. Results The level of IL-18 sharply decreased at the first day,but still higher than that of the low immunologic function group （ P 〈 0.05 - 0.001 ） , and arrived to the normal level at the 7 ~ day. The level of IFN-T always elevated over the normal group（ P 〈 0.05 ） and the low immunologic function group（ P 〈 0.01 ）. IL-4 decreased comparing with the that in normal group post the first day , but still higher than that of the low immunologic function group （ P 〈 0.0 5 - 0.001 ） , having no difference with the normal control group at 4^th day, elevated with the normal group 7^th day later （ P 〈 0.05）. Conclusion When BLAB/c mice infected the high or low dose CA,the major immunological reaction of the organism was Thl cytokines at early stage;The Th1/Th2 cytokines would elevated at the later stage,which showed the cellar immunity and humoral immunity took part in the response against the CA infection.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2006年第7期389-390,418,共3页
The Chinese Journal of Dermatovenereology
基金
江西省自然科学基金课题[0240016]
