摘要
目的通过定量检测粪乳铁蛋白水平,研究粪乳铁蛋白与炎症性肠病(IBD)的相关性。方法收集消化科和肠道门诊共177份新鲜粪便标本,其中活动期溃疡性结肠炎(UC)42例、静止期 UC 17例、活动期克罗恩病(CD)13例、静止期CD 5例、感染性肠炎41例、肠易激综合征(IBS)25例、健康人34例。用IBD-SCANTM试剂分别定量检测粪便中乳铁蛋白浓度。采用 Harvey-Bradshaw活动指数评分标准判断IBD是否活动,积分≥4为活动性。结果粪乳铁蛋白浓度分别为健康人(3．15 ±1．60)μg／g,IBS患者(2．54±1．49)μg／g,感染性肠炎患者(83．3±29．9)μg／g,活动期UC患者(1126．29 ±431．21)μg／g,活动期CD患者(1035．25±456．59)μg／g,静止期UC患者(96．58±82．46)μg／g,静止期CD患者(133．52±88．89)μg／g。IBS和健康人粪乳铁蛋白浓度差异无统计学意义(P>0．05);感染性肠炎与IBS和健康人粪乳铁蛋白水平差异有统计学意义(P<0．01)。粪乳铁蛋白在活动期IBD 中明显升高,与静止期IBD、IBS和感染性肠炎比较差异均有统计学意义(P<0．01)。粪乳铁蛋白在鉴别活动期与非活动期UC中的敏感性为90％,特异性为88％;鉴别活动期与非活动期CD中的敏感性为92％,特异性为80％。结论粪乳铁蛋白是一种敏感和特异的检测IBD是否活动的指标。IBD- SCANTM试验作为一种非侵入性筛选试验,为鉴别IBD和非IBD提供了有临床价值的手段,粪乳铁蛋白升高可排除IBS。
Objective To investigate the relationship between levels of fecal lactoferrin and inflammatory bowl disease （IBD） by IBD-SCANTM quantitative analysis. Methods One hundred and seventyseven fresh fecal samples were collected from the patients with IBD and the controls, including 42 cases of active UC, 17 inactive UC, 13 active CD, 5 inactive CD, 41 infective enteritis, 25 cases of irritable bowel syndrome （IBS） and 34 healthy volunteers. IBD-SCANTM was used to quantitatively determine the level of fecal lactoferrin. Harvey-Bradshaw active index was adopted to evaluate the activity of IBD, the score equal or higher than four was considered to be active 1BD. Results The level of fecal lactoferrin was （3. 15 ±1. 60）μg/g in healthy volunteers, （2.54± 1.49）/μg/g in IBS patients, （83.3 ±29.9）μg/g in infectiousenteritis, （l126.29±431.21）μg/ginactive UC, （1035.25±456.59）μg/ginactive CD, （96.58 ±82.46）μg/g in inactive UC and （133.52 ± 88. 89）μg/g in inactive CD, respectively. There was no difference between IBS patients and healthy volunteers （P〉0.05） in levels of fecal lactoferrin, but there was remarkable difference between the patients with infective enteritis and IBS or healthy volunteers. When IBD became active, the level of fecal lactoferrin increased significantly compared with that in inac- tive IBD, IBS and infective enteritis （P〈0.01）. The sensitivity and specificity of fecal lactoferrin in differentiating the active UC from inactive UC and the active CD from inactive CD was 90%, 92%and 88%, 80%, respectively. Conclusions Fecal lactoferrin is a sensitive and specific marker to evaluate the activity of IBD. As a non-invasive method, IBD-SCANTM appears to be efficient for differentiation of IBD from non-inflammatory bowel diseases. In addition, an increase of fecal lactoferrin can help to exclude IBS.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2006年第6期393-395,共3页
Chinese Journal of Digestion
关键词
乳铁蛋白
炎症性肠病
肠易激综合征
Lactoferrin
Inflammatory bowel disease
Irritable bowel syndrome
