摘要
AIM： To investigate c-met expression during early pancreatic carcinogenesis. METHODS： We used 46 bulk tissues and 36 microdissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative realtime reverse transcription-polymerase chain reaction. RESULTS： In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microclissected pancreatic cancer cells and pancreatitisaffected epithelial cells than in normal ductal epithelial cells （both, P 〈 0.01）. Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. CONCLUSION： Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.
瞄准：在早胰腺的致癌作用期间调查遇见 c 的表示。方法：我们使用了 46 体积纸巾和 36 件微把样品，包括正常的胰，慢性胰炎，和胰腺的癌症，为量的即时反向的抄写聚合酶链反应。结果：大批，组织分析，胰腺的癌症纸巾显著地表示了高水平比遇见 c 做了慢性胰炎和正常的胰纸巾。遇见 c 的层次没在慢性胰炎和正常的胰纸巾之间不同。在基于 microdissection 的分析，遇见 c 比在正常管的上皮细胞在微把的胰腺的癌症房间和影响胰腺炎的上皮细胞在高水平被表示(两， P < 0.01 ) 。有趣地，影响胰腺炎的上皮细胞表示了层次遇见 c 类似于那些胰腺的癌症房间。结论：Overexpression 遇见 c 在胰腺的致癌作用的早阶段期间发生，并且遇见 c 的表示的单个改变不为到胰腺的癌症房间的长期的影响胰腺炎的上皮细胞的前进是足够的。
基金
Supported by a Grant-in-Aid from the Ministry of Education,Culture, Sports, ScienceTechnology of Japan, Research Fellowships of the Japan Society for the Promotion of Science for Young Scientistsa grant from the Japanese Foundation for Research and Promotion of Endoscopy
