摘要
从患病鹅群中分离到一株禽副黏病毒,编号为2/GD/05/Go,简写为GPMV-2/05。经纯化后发现该病毒在透射电镜下呈圆形、大小比较均一,表面有纤突,对氯仿和酸敏感,不耐热,在中性和碱性溶液中比较稳定。血清学试验结果表明该病毒具有血凝活性,且能被鸡新城疫病毒(NDV)Ⅰ系、Ⅳ系抗血清部分抑制,但与禽流感、小鹅瘟等病毒无交叉反应。人工感染结果显示,新分离到的病毒对1、14、28、48日龄的鹅均有很强的致病性,尤以30日龄内最为严重,死亡率高达100%;经口服、点眼滴鼻、肌注、皮下注射等途径均能感染鹅,并且临床症状和死亡率与自然感染病例相似。该病毒对鸡也有很高的致病性和致死率,但用NDV强毒攻毒的鹅并不发病。通过免疫保护试验发现,GPMV-2/05与NDVⅠ系、Ⅳ系及强毒F48E9有部分免疫原性交叉。血清学、电镜观察、理化鉴定、病理组织学观察、免疫保护试验、RT-PCR鉴定以及致病性指数的测定等结果均表明该病毒是一株对鹅具有高致病力的禽副黏病毒。
A strain of avian paramyxovirus （GPMV-2/05） was isolated from goose in Guangdong province. Some infective characters of this strain, such as IVPI, ICPI and MDT, were determined. With virulent NDV F48E9 as control,the pathogenicity of GPMV-2/05 to geese of different ages （1,14,28 and 48 days old）was examined via oral, intranasal, intramuscular and hypodermic route of challenge, respectively. The results showed that the geese can not be infected by NDV F48Eg, however both geese and chicken were susceptible to GPMV-2/05 strain. According to the immunity protection test, we found that GPMV-2/05 could be prevented by the immunity of the NDV vaccine, which were NDV I vaccine, NDV Ⅳ vaccine and the virulent strain of NDV. On the basis of serological tests, electron microscopy,physiochemical properties and histopathological study, the virus was classified as a virulent avian paramyxovirus to goose.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第7期681-686,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(39770563)
广东省重大科技攻关项目(2005A20401003)
关键词
鹅
禽副黏病毒
感染
goose
avian paramyxovirus
infection
