摘要
目的:建立血浆中坎地沙坦的反相高效液相荧光检测方法。方法:采用 Agilent 1100高效液相色谱仪,色谱柱为 AgilentZorbax SB-C_(18)(4.6mm×150mm,5μm);以磷酸缓冲液(含0.1%磷酸,0.15%三乙胺)-乙腈(69:31)为流动相,流速1.0mL·min^(-1);柱温40℃;激发波长254nm,发射波长380nm。以厄贝沙坦作为内标。结果:血浆中坎地沙坦能得到较好的分离,血浆中杂质不干扰测定。坎地沙坦血浆线性范围为2.04～510ng·mL^(-1),标准曲线:Y=0.0048C+0.0006(r=0.9999),检测限为2.04ng·mL^(-1),方法回收率均接近100%。结论:本方法专属性强,灵敏度高,符合生物样品的分析要求。
Objective：To develop a sensitive and specific RP - HPLC method for determination of candesartan in human plasma. Methods：The RP- HPLC method was performed with Agilent Zorbax Eclipse SB- C18 （4. 6 mm × 150 mm ,5μm）. The mobile phase ,phosphate buffer [0. 1% H3PO4 and 0. 15% N（ C2H5 ） 3 ] - acetonitrile （69：31 ）, was used at a flow rate of 1.0 mL·min^-1. The temperature of column was 40℃. The fluorometric excitation and emission wavelengths were set at 254 nm and 380 nm, respectively. The internal standard was irbesartan. Results： The linear calibration curve was observed in the concentration range of 2. 04 -510 ng ·mL^-1. The linear regression equation was Y = 0. 0048 C ＋ 0. 0006 （ r = 0. 9999 ）. The lowest concentration of detection was 2. 04 ng·mL^-1. The relative recoveries were close to 100%. Conclusion ：The method was proved to be sensitive, and suitable for pharmacokinetic study of candesartan in human plasma.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第6期761-763,共3页
Chinese Journal of Pharmaceutical Analysis
