摘要
通过体外细胞培养方法，研究了进行舌鳞癌细胞株Ｔｃａ８１１３ｐ５３基因电穿孔法转染的最佳实验条件。结果显示在细胞培养至３６ｈ、电场强度为６２５Ｖ／ｃｍ时转染效果最佳，转染后选择剂Ｇ４１８的最佳浓度为３００μｇ／ｍｌ。按此条件已成功地进行了ｐ５３基因的体外转染。
The best conditions for p53 gene transfection in Tca8113 cell line by electroporation have been studied in vitro. The results showed that the effect reached the top when cell had been cultured till 36 hours and electric field intensity was 625 V/cm. The most suitable concentration of G418 which was used as selection agent was 300 μg/ml. The p53 gene had been successfully transfected based on these conditons.
出处
《华西口腔医学杂志》
CSCD
北大核心
1996年第3期180-182,共3页
West China Journal of Stomatology
基金
国家自然科学基金
美国纽约中华医学基金
关键词
口腔粘膜肿瘤
鳞状细胞癌
P53基因
基因转染
electroporation\ \ gene transfection\ \ oral squamous cell carcinoma\ \ p53 gene
