摘要
目的对从灵芝孢子粉中提取出来的纯多糖SGL-Ⅲ进行结构分析。方法多糖SGL-Ⅲ经比旋光、高效液相色谱法、苯酚-硫酸法确定物理性状后,再经部分酸水解、高碘酸氧化、Smith降解、甲基化分析及IR,GC,GC-MS和13C-NMR等方法确定其结构。结果SGL-Ⅲ相对分子质量为1.41×104,由葡萄糖(Glc)和半乳糖(Gal)组成,摩尔比为4.46∶1,为少分支结构,其基本结构中主链由(1→3)Glc和(1→6)Gal构成,分支点分别在6-O,2-O及4-O处,(1→4)Gal或(1→6)Glc位于侧链,分支末端残基为Glc。结论多糖SGL-Ⅲ具有天然活性多糖的特征,可以对其进行药理活性的研究开发。
OBJECTIVE To characterize the structure of homogeneous polysaccharide SGL-Ⅲ isolated from spores of Ganoderma lucidum. METHODS The physical characters of SGL-Ⅲ were determined by optical rotation test, HPLC and phenol-sulfuric methods. Then by means of partial hydrolysis with acid, periodate oxidation, Smith degradation, methylation, IR, GC, GC-MS and ^13C-NMR, the structure of polysaccharide SGL-Ⅲ was identified.RESULTS The molecular weight was estimated to be 1.41 ×10^4. Sugar compositional analysis of SGL-Ⅲ indicated that it was composed of glucose and galactose in the molar ratio of 4.46：1. SGL-Ⅲ was a low branched molecule. The main chain was made up of （1→3）-linked-Glc and（ 1→6）-hnked-Gal. Some of （1→3）-linked-Glc residues were substituted at 6-O and some of （1→6）-linked-Gal were substituted at 2-O and 4-O. The（ 1→4）-linked-Gal and （1→6）-linked-Glc were on the side chain. The branch end was Glc. CONCLUSION The polysaceharide SGL-Ⅲ shows some characters of bioactive polysaccharide. We will investigate its bioactivities in the further work.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第12期902-904,957,共4页
Chinese Pharmaceutical Journal
关键词
灵芝孢子粉
多糖
结构
spores of Ganoderma lucidum
polysaceharide
structure
