摘要
目的了解乳腺癌细胞系MCF-7中是否存在乳腺癌干细胞,以及在不同的培养条件下,干细胞池的变化情况。方法采用流式细胞技术检测在不同的培养条件下MCF-7中CD44和CD24的表达情况以及乳腺癌组织原代细胞中的CD44和CD24表达情况。结果MCF-7中存在乳腺癌干细胞,而且干细胞所占的比例会随培养过程中细胞数量的不断增加而逐渐下降;当化疗药物干预后导致细胞数量下降时,干细胞的比例则会明显上升。结论MCF-7中存在乳腺癌干细胞,而且MCF-7的干细胞池会随着微环境中生长信号的变化而变化。
Objective To know if MCF-7 breast cancer cell line has breast cancer stem cell,and change of stem cell pool in diverse incubation circumstance. Methods To mensurate the expression of CD44 and CD24 of MCF-7 in diverse incubation circumstance,and the expression of CD44 and CD24 of primary breast cancer cells from human breast cancer tissue by flow cytometry. Resuits Breast cancer stem cell persists in the MCF-7 breast cancer cell line. The proporation of stem cell gradually decrease with the number of MCF-7 gradually increasing in the process of culture,and the proporation of stem cell markedly increases after chemotherapy. Conclusion Breast cancer stem cell persists in the MCF-7 breast cancer cell line, and stem cell pool of MCF-7 changes with growth singal in cellular environment.
出处
《实用癌症杂志》
2006年第2期126-129,共4页
The Practical Journal of Cancer
关键词
干细胞
乳腺癌
体外培养
化疗
Stern cell
Breast cancer
Culture in vitro
Chemotherapy
