摘要
以海参肽得率为指标,分别考察了酶解加酶量、时间、pH、温度、底物浓度对胰蛋白酶酶解海参蛋白质反应的影响。通过正交实验确定了胰蛋白酶酶解海参蛋白的最佳工艺条件:酶加量6 000 U/g,水解反应时间3 h,温度55℃,pH 8.0,底物浓度1.0%,在此条件下,海参肽得率可达32.93%。
Taking the ratio of holothurian peptides as an index, the effect of the added amount of trypsinase, time, pH, temperature and substrate concentration on the enzymolysis of holothurian were studied. The optimum hydrolytic condition of trypsinase is determined by orthogonal experiments as follows： amount of enzyme added, 6 000 U/g; reaction time, 3 h temperature, 55℃; pH, 8.0; substrate concentration, 1.0 %. Under this condition, the ratio of holothurian peptides achieves 32.93%.
出处
《大连轻工业学院学报》
2006年第2期83-85,共3页
Journal of Dalian Institute of Light Industry
基金
国家自然科学基金资助项目(30571449)
关键词
海参
肽
胰蛋白酶
holothurian peptide
trypsinase
