摘要
目的通过对幽门螺杆菌微球免疫后Balbc小鼠血清IgG、IgA、胃肠sIgA的检测及脾脏IL4、IFNγ的mRNA表达水平、IgGASC、IgAASC数量的比较观察,探求疫苗的免疫保护机理。方法取Balbc小鼠口服免疫疫苗,剂量为150μg/(只·次),免疫时间为0、14、30d。2周后取血清及胃肠洗液进行抗体检测,同时取脾脏检测细胞因子mRNA表达水平及ELISPOT检测抗体分泌细胞数量变化。结果免疫后小鼠以上检测指标与对照组比较均有不同程度的升高。结论微球疫苗所诱发的免疫保护是细胞免疫、体液免疫两者共同作用的结果。
Objective To study the immunoproteeive mechanism of helicobacter priori （Hp） microsphere vaccine. Methods Mice were immunized with lip vaccine on 0, 14,and 90 d, and the dose of vaccine for every mouse was 150 μg every time. The mice were sacrificed the samples used to detect the antibody levels after 2 weeks. The levels of IgG and IgA in serum, and the slgA in gastric and intestinal juice were detected by ELISA. The level of IL-4 and IFN-γ mRNA in spleen were detected by RT-PCR. The quantity of IgG-ASC and IgA-ASC in spleen were detected by ELISPOT. Results The detected antibody levels in immunized mice were increased obviously. Conclusion The resuits suggest that the immune responses in the Hp microsphere vaccine-immunized mice are induced by the body fluid and the cell immune response together.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第4期384-386,共3页
Immunological Journal
基金
国家"十五"863课题资助(2001AA215161
2003AA21502)
关键词
微球疫苗
幽门螺杆菌
免疫应答
Microsphere vaccine
Helicobacter pylori
Immune response
