摘要
目的:探讨建立与人类脑出血(intra-cerebral hemorrhage,ICH)相似、稳定可靠、重复性好的犬ICH模型的可行性.方法:选择健康家犬45只,经股动脉插管DSA下,导丝经导管进入并刺破大脑中动脉.制作ICH模型,以成功后2h脑CT检查结果确定血肿部位并计算血肿体积,观察24h内血肿体积变化及犬死亡情况.结果:45只犬脑出血部位均在基底节区或临近颞叶;血肿体积2.1～4ml者明显多于4.1～5ml、1.5～2ml及≥5ml者(P均＜0.01);体积为4.1～5ml者明显多于1.5～2ml及≥5ml者(P均＜0.01);血肿≤4ml破入脑室者明显少于≥4.1ml者(P＜0.01),前者24h无1只死亡,明显少于后者(P＜0.01).结论:DSA介入下制作犬ICH模型,血肿部位合适、大小适中、模型稳定、破入脑室及死亡率均低,更接近于人类ICH.
Objective：To prepare stable, reliable, reproducible and similar human intra-cerebral hemorrhage （ICH） model in dogs. Methods：45 healthy domestic dogs were performed with femoral arterial catheterization with digital subtraction angiography （DSA） intervention method. The guide wire was placed and the middle cerebral artery was pnicked thus dog model of ICH was prepared. Two hams later it was examined with computed tomography to ensure hematoma positon and calculate its volume; and the change of hematoma volume and death rate of dogs were observed. Results： ICH in 45 dogs were all located at basal segment region or near temporal lobe; the number of dogs with a hematoma volume 2.1 - 4ml was lager markedly than that of dogs with a hematoma volume 4.1 - 5ml, 1.5 - 2ml and ≥ 5ml （ all P 〈 0.01 ） ; the number of dogs with a hematoma volume 4.1 - 5ml was lager markedly than that of dogs with a hematoma volume 1.5 - 2ml and ≥ 5ml （all P 〈 0.01 ）;the dogs with a hematoma volume ≤ 4ml which broken into ventricles of brain were less markedly than that ≥4.1ml （ P 〈 0.01 ）. In the former , no dogs died within 24h, which was less markedly than that in the latter （ P 〈 0.01 ）. Conclusion： Preparation of dog model of ICH with DSA intervening metliod is stable and reliable; size of hematoma is moderate; rate of breaking into ventricles of brain and death rate are low, and it is similar to human being.
出处
《医学影像学杂志》
2005年第12期1092-1095,共4页
Journal of Medical Imaging
基金
鲁科成(2004-760)
枣庄市学科带头人资金资助项目(2004-26)
关键词
犬脑出血模型
数字减影血管造影
体层摄影术
X线计算机
Dog model
Intra-cerebral hemorrhage
Digital subtraction angiography （DSA）
Tomography, X- ray computed
