摘要
报道了地塞米松诱导大鼠胸腺、脾细胞凋亡机制的初探。结果表明，地塞米松与这些器官的细胞共同培养不同时间，其糖皮质激素受体（ＧＣＲ）量发生变化，胸腺细胞ＧＣＲ量在培养１．５ｈ组为６７．７２±１５．２１（ｆｍｏｌ／１０６细胞），显著高于对照组３０．５７±８．２５及５ｈ组３６．９１±９．１７（Ｐ＜０．０５），脾细胞ＧＣＲ量各组差异不显著（Ｐ＞０．０５）。在细胞周期蛋白依赖抑制因子（ρ１６）实验中，观察到Ｐ１６阳性细胞数在１．５ｈ组多于对照组及５ｈ组。在形态学及生化实验中，对照组及１．５ｈ组，胸腺、脾细胞Ｇｉｅｍｓａ染色形态一致，其ＤＮＡ未见断裂带；而５ｈ组，胸腺：脾细胞可见核固缩、深染、核碎裂、体积小的凋亡细胞，其ＤＮＡ电泳可见典型“梯状”条带。由此，推测地塞米松通过ＧＣＲ的作用，影响细胞周期相关蛋白，进而诱导免疫细胞凋亡。
Reported the machenism of thymic and splenic cell apoptosis induced by dexamethasone.The results showed that when the immunologic cells were cocultured with dexamethasone at different time, the glucocorticoid receptor(GCR) in that cells changed greatly. GCR content of thymocytes at 1.5h culture was 67.72±15.21(fmol/10￣6cell) which was higher than that of control group 30. 57±8. 25 and 5 h group 36. 91±9. 17(P<0.05). No great changes of GCR content in splenic cell were found among that groups(P>0. 05).In the test of cyclins dependant inhibitor-p16, found that the number of p16 positive cells at 1.5h group was higher than that of control and 5h group.In the cellular morphologic observation and DNA biochemical analysis, the morphologic changes of immunologic cells at control and 1.5 h group were the same and the DNA from that cells showed no 'ladder'alternation in electrophoresis. However, the cells at 5h group were keryopyknosis, condesation of cell and the DNA from those cells showed typically 'ladder' apperance, Therefore, conclude that the dexamethasone might through its GCR affect cell cycle and induce immunological cell apoptosis.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1996年第4期222-224,共3页
Chinese Journal of Immunology
关键词
地塞米松
免疫细胞
细胞凋亡
Dexamethasone Immunological cell Apoptosis
