摘要
目的研究白细胞介素1B基因(IL-1B)启动子区域-31位点和-511位点及白细胞介素1受体拮抗剂基因(IL-1RN)多态性在我国北方人群胃癌患者与胃炎患者中的分布,探讨各基因型与胃癌的相关性。方法收集126例胃癌患者与125例慢性胃炎患者(对照组)的外周血标本和流行病学资料,提取基因组DNA；IL-1RN基因采用PCR方法直接测定,IL-IB-31基因采用PCR-CTPP方法,IL-1B-511基因采用PCR-RFLP的方法进行基因分型。通过快速尿素酶、^(14)C呼气试验及Hp血清IgG抗体的方法检测Hp感染。结果IL-IRN有5种基因型,分别为1/1、1/3、1/4、1/2和2/2型,其出现频率在胃癌组中分别为76．19%、4．76%、6．35%、11．90%和0．79%；在对照组分别为76．00%、4．00%、4．80%、13．60%和1．60%。各基因型在胃癌组和对照组中分布差异无统计学意义。IL-1B-31位点有3种基因型C/C、C/T和T/T型,在胃癌组中的频率分别为12．70%、47．62%和39．68%；在对照组中的频率分别为28．00%、48．80%和23．20%。与C/C型相比较,携带T/T基因型者胃癌发生的风险增加,OR=3．772(95%CI=1．786-7．966)。IL-1B-511位点有3种基因型C/C、C/T和T/T型,在胃癌组中的频率分别为19．20%、56．80%和24．00%；在对照组中的频率分别为23．38%、49．19%和27．42%。各基因型在胃癌组和对照组中分布差异无统计学意义。结论IL-1B基因启动子区域-31位点的基因多态性可能与国人胃癌易感性相关；尚未有证据表明IL-1RN和IL-1B-511位点的基因多态性与国人胃癌易感性相关。
Objective To evaluate the association between interleukin-1 （ IL-1 ） loci polymorphisms and increased risk of gastric carcinoma in samples from northern Chinese population. Methods Blood samples from 126 patients with gastric cancer and 125 controls with chronic gastritis were collected. Genomic DNA was extracted and polymorphisms at -31（C to T）, -511 （C to T） and at intron 2 （86-bp VNTR） of IL-1RN were genotyped by PCR-CTPP, PCR-RFLP and PCR. For detection of Hp infection fast urenase test, ^14C breath test and serum anti-Hp IgG antibody assay were used. Results Five kinds of polymorphism of IL-1RN were found as 1/1,1/3, 1/4-, 1/2 and 2/2, and the frequencies in patients were 76. 19%, 4. 76% ,6. 35% ,11.90% and 0. 79%, respectively. However, the frequencies in controls were 76. 00% , 4. 00% ,4. 80% ,13.60% and 1.60%. No significant differences were observed between cases and controls in each genotype. The polymorphism of IL-1B-31 allele was C/C, C/T and T/T. The frequencies in patients were 12.70% ,47. 62% and 39. 68% ,and in controls 28.00% ,48. 80% and 23.20% respectively. IL-1B- 31 T/T carriers were at an increase risk of gastric cancer with an odds ratio of 3. 772（95% CI, 1. 786 - 7. 966）. IL-1B-511 alleles were C/C, C/T and T/T. The frequencies in patients were 19. 20%, 56. 80% and 24. 00% and in controls, 23. 38%, 4-9. 19% and 27. 42% respectively. No significant differences were observed between cases and controls in each genotype. Conclusion In Chinese population, the polymor-phism of IL-1B-31 alleles may be associated with the susceptibility of gastric cancer. However, no evidence was found to support that the polymorphisms of IL-1RN and IL-1B-511 alleles had relationship with gastric cancer.
出处
《中华消化内镜杂志》
2006年第3期189-193,共5页
Chinese Journal of Digestive Endoscopy
关键词
螺杆菌
幽门
多态性
基因
胃癌
Helicobacter pylori
Polymorphism,genes
Gastric cancer
