摘要
目的了解IL-1B及IL-1RN基因多态性在河南汉族人群中的分布特点及其在不同地区的异同。方法用PCR和限制性内切酶图谱的方法检测河南地区243名人群中IL-1B-31、IL-1B-511及IL-1RN基因多态性,并结合文献进行不同地区间的比较。结果在河南汉族人群中,IL-1B-31等位基因C型发生频率高(0.59),T型为0.41;IL-1B-511等位基因T型较高(0.56),C型为0.44;IL-1RN等位基因频率分别为I型(0.899)、Ⅱ型(0.078)、Ⅲ型(0.002)、Ⅳ型(0.02),Ⅰ型纯合子最为常见(占82%),其次为Ⅰ/Ⅱ型(占12%)。与其他地区相比,基因频率的分布国内和东亚地区较为接近,而和西方国家不同。结论IL-1B-31I、L-1B-511I、L-1RN基因多态性在不同地区人群间的分布存在明显差异。
Objective To identify the distribution of IL-1B and IL-1RN gene polymorphisrns in Henan Han population, and study the difference between Henan and other regions. Methods PCR- RFLP was used to analyze the IL-1B-31 and IL-1B-511 polymorphisms, the VNTR polymorphism of IL-1RN was examined with PCR, then compared with different regions. Results It showed that in Henan Han population, the IL-1B-31 C gene frequency was the most frequent one （0.59）, T gene frequency was 0.41; the IL-1B-511 T gene frequency was the most frequent one （0.56）, C gene frequency was 0.44; the genotype frequency of IL-1RN was 0.899 for Ⅰ allele, 0.078 for Ⅱ allele, 0.002 for Ⅲ allele, 0.02 for Ⅳ allele, the IL-1RN Ⅰ allele was the highest one. The gene frequency of IL-1RN Ⅰ/Ⅰ was the most frequent one （the proportion was 82% ）, While the second one was the IL-1RN Ⅰ / Ⅱ （the proportion was 12% ）. Compared with other regions, the gene frequencies of IL-1B-31, IL-1B- 511, IL-1RN in Henan Han population were almost similar with east-Asia, and different with the west countries. Conclusions Distribution of interleukin-lB and interleukin-1 RN gene polymorphisrns were variable in different population.
出处
《疾病控制杂志》
CAS
2006年第3期226-229,共4页
Chinese Journal of Disease Control and Prevention
