摘要
目的:研究芝麻素对肝脏的保护作用。方法:1.以扑热息痛制备小鼠急性肝损伤模型,给予不同剂量芝麻素,测定给予芝麻素后动物血清ALT、GST浓度及肝匀浆SOD、MDA、GSH含量并比较其差异。2.以饮用酒制备大鼠亚急性肝损伤模型,给予不同剂量芝麻素,测定并比较各组动物血清ALT、AST、GST浓度、肝匀浆SOD、MDA、GSH含量及肝脏病理改变。3.以四氯化碳制备大鼠慢性肝损伤模型,最后1个月同时给予芝麻素,测定各组动物血ALT、AST、GST浓度、A/G比值、肝匀浆SOD、MDA、GSH、羟脯氨酸含量,计算肝脏脏器系数,观察肝脏病理改变,并比较各组间差异。结果:⒈芝麻素可抑制APAP所致小鼠血清ALT、GST水平的升高,降低肝匀浆MDA含量,增加肝匀浆GSH含量(P<0.05或P<0.01)。⒉芝麻素用药10d,可抑制酒精所致大鼠血清ALT、AST、GST水平的升高,降低肝匀浆MDA含量,增加肝匀浆GSH含量(P<0.05或P<0.01)。病理学检查提示,芝麻素用药使模型动物肝细胞肿胀、脂肪变性及炎性浸润明显减轻。⒊芝麻素用药1m,可抑制CC l4所致大鼠血清ALT、AST、GST水平的升高,降低肝匀浆MDA含量,增加肝匀浆GSH含量,增强肝SOD活性(P<0.05或P<0.01)。使模型动物肝脏器系数减小,A/G比值上升,羟脯氨酸含量下降(P<0.05或P<0.01)。病理学检查提示,芝麻素用药使模型动物细胞肿胀、脂肪变性和炎性浸润明显减轻,纤维化倾向改善。结论:芝麻素可显著改善APAP引起的急性肝损伤,减轻酒精所致的亚急性肝损伤,降低CC l4对肝脏的慢性损伤,抑制或延缓肝脏的纤维化。
Aim： To investigate the protective effects of sesamin against liver damage in rodents. Methods： 1. Paracetamol was employed to build the acute mouse liver injury model. Before administration of APAP, sesamin was given intragastrically in the treated groups. At the end of test, concentrations of ALT and GST in serum, activity of SOD and the contents of GSH and MDA in liver homogenate were detected. 2. Alcohol was used to set up the sub-acute rat liver injury model. At 30 min before the first alcohol administration every day, sesamin was given intragastrically in treated group rats. After last administration, rats were sacrificed. Concentrations of ALT、AST and GST in serum, activity of SOD and contents of GSH and MDA in liver homogenate were detected. Liver was excised and fixed in formalin for histopathological examination. 3. Carbon tetrachloride was used to build the chronic liver injury model in rat and sesamin was intragastrically administrated once a day since the first day of the third month. After the last administration, rats were sacrificed. Concentrations of ALT, AST and GST and contents of albumen （A） and globulin （G） in serum, the activity of SOD, contents of GSH and MDA in liver homogenate were detected. Liver was weighed to calculate the organ coefficient and was taken for histopathological examination. Results： 1. For the liver damage animals induced by APAP, sesamin could significantly inhibit the elevation of ALT, GST levels in serum and MDA content in liver homogenate, and could increase the content of GSH in liver homogenate. 2. For the liver damage animals induced by alcohol, sesamin could significantly inhibit the elevation of ALT, AST and GST levels in serum and MDA contents in liver homogenate. Contents of liver GSH were also increased after sesamin administration. Histopathological examination showed that sesamin could ameliorate the inflammatory infiltration, the cellular swelling and the fatty degeneration changes in liver. 3. For the liver damage animals induced by CCl4, sesamin could significantly inhibit the elevation of ALT, AST and GST levels in rat serum and MDA content in liver homogenate. Contents of liver GSH and activities of SOD were increased after sesamin dosing. The A/G ratio and contents of hydroxyproline in liver were decreased. Contents of hydroxyproline in liver homogenate and liver coefficient decreased, too. Histopathological examination showed that the inflammatory infiltration, cellular swelling, fatty degeneration and fibrosis in liver were ameliorated. Conclusion： Sesamin showed a marked protective effect against liver damage caused by APAP, alcohol and carbon tetrachloride.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2006年第3期27-32,共6页
Pharmacology and Clinics of Chinese Materia Medica
关键词
芝麻素
扑热息痛
酒精
四氯化碳
肝损伤
抗氧化
sesamin
paracetamol
alcohol
carbon tetrachloride
hepatic injury
anti-oxidation
