摘要
根据MADS-box基因保守区结构,设计简并引物,从板栗(Castaneamollissima)中分离出花特异表达基因的cDNA片段。并通过5’RACE方法获得了全长cDNA,命名为CmMADS3。该片段全长1016bp,包含一个729bp的开放阅读框,推导的氨基酸序列(243个氨基酸)与拟南芥的SEP1、SEP2和SEP33类MADS-box蛋白有很高的序列相似性。系统进化分析同样将CmMADS3基因归入MADS-box基因家族的AGL2组。RT-PCR分析显示,该基因在板栗的花和幼果中表达丰度高,在茎中有微弱的表达,在叶中不表达,研究结果表明CmMADS3基因是板栗花器官发育中具有E功能的功能基因。
As a kind of transcription factors, MADS-box genes play an important role in various plant development processes, especially in the development of floral organs. The flower specific gene of Castanea mollissima, named CmMADS3 （ GeneBank accession number DQ148295 ）, was cloned from floral organs by the method of 5＇ RACE using degenerate primer designed according to the MADS-box gene family conserved region. The fragment was 1016 bp long and conrained an open reading frame of 729 bp coding a polypeptide of 243 amino acids. Homology analysis showed that the deduced CmMADS3 protein was highly homologous to the MADS-box genes SEP1, SEP2 and SEP3 of Arabidopsis thaliana. Phylogenetic analysis also indicated that CmMADS3 belonged to the AGL2 group of MADS-box gene family. RT-PCR analysis showed that CmMADS3 expressed at high levels in flowers and young fruits and at low levels in sterns. No CmMADS3 expression was detected in leaves. Our result suggested that CmMADS3 was related to E-class genes in the development of
出处
《云南植物研究》
CSCD
北大核心
2006年第3期295-299,共5页
Acta Botanica Yunnanica
