摘要
大肠杆菌(Escherichia coli)通过二信号传导体系RcsC-RcsB能够激活cps(capsule polysaccharide synthesis)基因群,在细胞表面形成一层粘性的保护性荚膜,使大肠杆菌具有较强的致病性。本文通过将基因组文库导入大肠杆菌,筛选到了名为acnA的基因,它编码合成乌头酸酶,在细胞内三羧酸循环和乙醛酸循环代谢中起关键作用,多拷贝的acnA能有效地抑制cps基因群的表达。
RcsC-RcsB two-component system can stimulate the expression of cps(capsule polysaccharide synthesis) genes in Escherichia coli,forming a sticky capsule in cell surface,which associates with pathogenesis.By transferring genomic library into Escherichia coli,we are able to screen out a gene named acnA which can code into an aconitase.The multicopy of acnA can represse the expression of cps effectively.
出处
《河南科技大学学报（自然科学版）》
CAS
2006年第3期76-78,共3页
Journal of Henan University of Science And Technology:Natural Science
基金
天津大学自然科学基金项目(20050201)
