摘要
用原子力显微镜（ＡＦＭ）研究了磷脂ＤＭＰＣ三层Ｌａｎｇｍｕｉｒ－Ｂｌｏｄｇｅｔｔ（ＬＢ）膜的分子排列结构，结果表明：在磷脂ＬＢ膜的两相（液体压缩相Ｌｉｑｕｉｄ－ｃｏｎｄｅｎｓｅｄｐｈａｓｅ和液体扩张相Ｌｉｑｕｉｄ－ｅｘｐａｎｄｅｄｐｈａｓｅ）共存时，液体压缩相中的磷脂分子排列紧密，取向一致，分子间作用力较大，因而能够得到分子图像。而液体扩张相中的磷脂分子排列松散，取向混乱。分子间的作用力较弱，难于得到分子图像。在液体压缩相中磷脂分子以单斜晶格结构排列，分子间隔为０．７２ｎｍ．分子高度为２．１ｎｍ。这一结果和ＤＭＰＣ的单晶结构进行了比较。
The molecular anagelnent of phosphoilid Langnuir-BlodgCtt theyer films investigated by using atomal form microscopy. The adults indicated that in the coexistence ofliqed-condensed phase and liquid-expanded phase, the phospholipid molecules in liquid-condensed phase packed tightly. The intermolecular intelaction is saving enough tO obtainmolecular alga of the fAn. The phosphohpid molecules in liqed expanded phase, however,packed disorderly. It was difficult to obtain a molecular adage for the weak intheedve foal between adjaCent molecules in LE phase. The fAn in liquid-expanded phase was systematicallydestroyed for the hp interaction in boging at molecular scale. In liqind-condenSed Phase,phosphompid molecules were organed in rnonocthe lattiCe with mol￣ spacing of 0.72urnand rmed molecular height of 2.1nm. The results were discussed in comparison with singleoptal structare.
出处
《生物物理学报》
CAS
CSCD
北大核心
1996年第1期67-70,共4页
Acta Biophysica Sinica
基金
国家自然科学基金
关键词
磷脂
生物膜
LB膜
晶格结构
原子力显微镜
Phospholipid
Langmuir-Blodgett film
Lattice structure Atomic force microscopy
