摘要
目的:观察依益气活血化瘀、清热平肝、熄风定惊法立方制成康脑液对大鼠脑缺血及脑缺血再灌注时软脑膜微血流量的干预作用。方法:实验于2004-09/12在河北北方学院医学院病理生理实验室完成。①选用清洁级雄性Wistar大鼠20只,鼠龄3个月。按随机抽签法将大鼠分为2组:治疗组和模型组,每组10只。治疗组:灌胃康脑液(主要成分为黄芪、丹参、川芎、葛根、钩藤、三七粉等)。制备:常规煎煮2次,再将两煎的上清液混合,文火煎浓缩至70mL),2mL/次,1次/d(相当于给原生药3.8g)。模型组:灌胃等量生理盐水。②2组均在灌胃第18天的给药后2h用颈动脉引流法复制大鼠脑缺血再灌注损伤模型。右侧颞顶部造一个5mm×8mm开放式颅窗,以MKD-1型多功能激光多普勒微循环血流仪分别记录造模前,脑缺血后15,30,45,60,75,90min及再灌注后15,30,60,90,120,150,180min软脑膜的微血流量。结果:Wistar大鼠20只均进入结果分析。①模型组大鼠脑缺血后软脑膜微血流量骤减,随着缺血时间的延长逐渐有所回升,但始终明显低于造模前(P<0.01)。治疗组大鼠脑缺血后15～30min软脑膜微血流量也明显减少(P<0.05～0.01),缺血后45min,其绝对值虽然也未恢复至实验前水平,但差异不明显(P>0.05)。治疗组脑缺血15～60min软脑膜微血流量明显多于模型组(P<0.05～0.01)。②模型组大鼠脑缺血再灌注后30min内软脑膜微血流量回升较为明显,30～90min微血流量再次降低,之后又回升,但始终明显低于造模前(P<0.01)。治疗组大鼠脑缺血再灌注后180min内,软脑膜微血流量绝对值低于造模前,但差异不明显(P>0.05)。治疗组大鼠再灌注后60～90min及150min时软脑膜微血流量明显多于模型组(P<0.05～0.01)。结论:康脑液能拮抗大鼠脑缺血及再灌注时软脑膜微血流量的减少,起到脑保护作用。
AIM： To observe the interventional action of self-made Kangnaoye （made based on tonifying qi,promoting blood flow, removing blood stasis, clearing heat, calming the liver, stopping endogenous wind and arresting convulsion） on regional cerebral blood flow （rCBF） with cerebral iachemia and reperfusion in rats.
METHODS： The experiment was conducted at Laboratory of Pathophysiology, Medical College, Hebei North University from September to December 2004. ①Totally 20 clean-grade male Wistar rats aged 3 months were selected and randomly assigned into the two groups： treatment group and model control group with 10 rats in each group. The rats in treatment group were treated with Kangnaoye （composed of the astragalus, danshen root, lignstrazine hydrochloride, kudzuvine root, gambir plant, sanchi and so on. Preparation： routinely decacted for twice. Supernatant liquid was mixed and decacted by 70 mL at a simmer） by gastric peffusion, 2 mL every time, once a day （equal to 3.8 g crude drug in whole）. The rats in the model group were treated with saline of the same volume. ②At day 18 after gastric perfusion rat models with cerebral ischemia-reperfusion injury were reproduced with carotid artery drain 2 hours after drug administration in the two groups. Opened skull windows （5 min×8 mm） were performed at right temple to investigate the rCBF before establishing models, after cerebral iachemia for 15, 30, 45, 60, 75, 90 minutes and after reperfusion for 15, 30, 60, 90, 120, 150, 180 minutes.
RESULTS： A total of 20 Wistar rats were involved in the result analysis. ①The rCBF decreased after cerebral iachemia in rats of the model group, and increased gradually with the prolongation of iachemie time. but still was significantly lower than that before establishing models （P 〈 0.01 ）. The rCBF markedly reduced in the treatment group after cerebral iachemia for 15-30 minutes （P 〈 0.05-001 ）. 45 minutes after isehemia, its absolute value did not recover to that before experiment, but the difference was insignificant （P 〉 0.05）. The rCBF was remarkably more in the treatment group than that in the model group at cerebral isehemia for 15-60 minutes （P 〈 0.05-0.01 ）. ②The rCBF at 30 minutes after cerebral iachemia-reperfusion in the model group obviously increased. From 30-90 minutes the rCBF decreased again, and than increased gradually, but still was dramactically lower than before establishing models （P 〈 0.01）. The absolute value of rCBF within 180 minutes after cerebral ischemia-reperfusion of rats in the treatment group was lower than that before establishing models, but the difference was insignificant （P〉 0.05）. The rCBF of rats in the treatment group after reperfusion from 60-90 minutes and 150 minutes was distinctly more than that in the model group （P 〈 0.05-0.01 ）.
CONCLUSION： Kansnaoye can obviously improve the rCBF in rats with cerebral ischemia and reperfusion injury and play a protective effect on brain.
出处
《中国临床康复》
CSCD
北大核心
2006年第23期46-48,共3页
Chinese Journal of Clinical Rehabilitation
基金
河北省卫生厅资助项目(03030)~~
