摘要
靶向治疗系统是目前研究的热点,用微乳化-离子交联方法制备包覆阿霉素的碳包铁/海藻酸钠复合纳米微球,以水溶性碳二亚胺为交联剂,将载药微球与单抗Hab18连接,制备出了免疫磁性药物纳米微球。对该免疫磁性微球的理化性能进行了表征,同时检测了免疫磁性微球中抗体的活性和免疫磁性微球与靶细胞的体外结合情况,结果表明,免疫磁性药物纳米微球平均粒径约为171.2nm,外观为球型,铁含量为14.6%,载药量为10.8%,且具有强磁响应性和长时间药物缓释效果。同时在体外该微球能够与靶细胞特异性结合。这种免疫磁性药物纳米微球有望成为一种优良的靶向肿瘤药物载体。
Immunomagnetic drug loaded microspheres （IMDM） were prepared by cross-linking the monoclonal antibody Habl8 to alginate magnetic doxorubicin microspheres. The morphology of IMDM was characterized by transmission electron microscopy. The size distribution of IMDM was determined by laser light scattering. The amount of Fe and drug containing in the microspheres were examined respectively. Flow cytomctry was used to evaluate the immunological activity of antibody on IMDM, ELISA method was used to evaluate the specific combination between IMDM and human liver tumor SMMC-7721 cells. The results showed that IMDM had spherical morphology, the average size was approximately 171.2 ± 2.21nm. The Fe content in the IMDM was 14.6%, and the drug loading was 10.8%. IMDM had a good response to the magnetic field and had long time drug releasing property. The immunological activity of antibody conjugated with IMDM was well remained ; IMDM could also combine with SMMC- 7721 cells, IMDM with magnetic and specific targeting ruction could be a promising carriers for cancer therapy.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2006年第3期305-309,共5页
Chinese Journal of Biomedical Engineering
基金
广东省自然科学基金重点项目(No.04006966)
广东省卫生科研基金(No.A2003697)。
