摘要
建立了HPLC-MS法测定人血浆中茴拉西坦代谢产物对甲氧基苯甲酰胺基丁酸(2)的浓度。以N,N-二甲基-3,4-亚甲氧基苯乙胺(3)为内标,采用Thermo C18色谱柱,流动相为30mmol／L乙酸铵溶液(含0．5％甲酸和0．01％三氟乙酸)-乙腈(84:16),HPLC-MS采用ESI+电离源,选择正离子检测模式,检测的离子为m／z 135(2,M+H)和163(3,M+ H)。2在0．058～14．03μg／ml浓度范围内线性良好,回收率在90％以上。18名健康志愿者口服茴拉西坦胶囊0．2g后的药动学参数AUC0→t、AUC0→∞、tmax、Cmax和t1/2分别为(9．21±1．72)μg·h·ml-1、(9．30±1．75)μg·h·ml-1、(0．47±0．11)h、(9．44±2．13)μg／ml和(0．66±0．10)h。
A HPLC-MS method for determination of metabolite of aniracetam -- p-anisamidobutyric acid （2） in human plasma was established. N,N-Dimethyl-3,4-methylenedioxy-methamphetamine （3） was used as internal standard. A Thermo C18 column was used with the mobile phase of 30mmol/L ammonium acetate solution （containing 0.5 % formic acid and 0.01% trifluoroacetic acid） -acetonitrile （84： 16）. The analytes were ionized in the electro spray ionization （ESI^＋） ion source of the mass spectrometer and detected in the selected ion monitoring （SIM） mode. SIM ions were m/z 135 （M＋H） for 2 and 163 （M＋H） for 3. The calibration curve of 2 A was linear in range of 0.058 - 14.03μg/ml. The recovery was above 90%. Eighteen volunteers were orally administrated 0.2g of aniracetam, the pharmacokinetic parameters were A UC0→t （9.21 ± 1.72） μg·h·ml^-1, AUC0→∞= （9.30 ± 1.75） μg·h·ml^-1, tmax （0.47 ± 0.11 ） , Cmax （9.44 ± 2.13） μg·ml^- 1 and t1/2 （0.66 ± 0.10） h, respectively.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2006年第6期418-420,共3页
Chinese Journal of Pharmaceuticals
