摘要
目的探讨组织因子能否诱导人脐静脉内皮细胞表达巨噬细胞炎性蛋白1α,以阐明其在动脉粥样硬化发生中的作用。方法体外培养人脐静脉内皮细胞,分组加入不同浓度的组织因子,采用酶联免疫吸附测定法巨噬细胞炎性蛋白1α蛋白的表达,用逆转录聚合酶链反应检测巨噬细胞炎性蛋白1αmRNA的表达,并用单核细胞粘附率试验检测单核细胞粘附。结果组织因子呈剂量依赖性增加内皮细胞表达巨噬细胞炎性蛋白1α蛋白,0.01、0.10、1.00及10.00 nmol/L组织因子分别使巨噬细胞炎性蛋白1α表达明显增加127%±14%、151%±11%、196%±13%及267%±43%。同时观察到组织因子促使单核细胞粘附率增加,并且粘附率与加入的组织因子浓度呈正相关。逆转录聚合酶链反应发现组织因子能促使巨噬细胞炎性蛋白1αmRNA表达明显增加。结论组织因子能诱导内皮细胞表达巨噬细胞炎性蛋白1α增强,这可能与组织因子致动脉粥样硬化作用机制有关。
Aim To investigate the effects of tissue factor(TF)on the expression of macrophage inflammatory proteinin-1αin cultured human umbilical endothelial cell(hUVEC)Methods Different concentrations of tissue factor were incubated with hUVEC.Cell enzyme linked immunosorbent assay(ELISA)was used to detect the expression of ma(rophage inflammatory protein-1α.Monocyte adhesion assay was used to detect the monocyte adhesion,ma(rophage inflammatory protein-1αmRNA expression were determined by reverse transcfiptase-polymerase chain reaction(RT-PCR).Results Tissue factor dramatically enhanced the levels of ma rophage inflammatory protein-1αin a dose dependent manner,increasing ma(rophage inflammatory protein-1αprotein expression in hUVEC by 127%±14%at 0.01 nmol/L,151%±11%at 0.10 nmol/L,196%±13%at 1.00 nmol/L and 267%±43%at 10.00nmol/L.In addition,10.00nmol/L of TF for 2 hours could significanfly increase the amount of monocyte adherence to endothelial cell.Moreover,RT-PCR analysis demonstrated that the amount of ma(rophage inflammatory protein-1αmRNA was increased after treatment withTF for 24 hours.Conclusion Tissue factor can induce ma(rophage inflammatory protein-1αexpression in hUVEC,which may thereby influence the pathogenesis of atherosclerosis.
作者
沈琴
朱铁兵
杨志健
潘金顺
曹克将
马文珠
SHEN Qin;ZHU Tie-Bing;YANG Zhi-Jian;PAN Jin-Shun;CAO Ke-Jiang;and MA Wen-Zhu(The Cardiology Departnent of the First Affiliated Hospiutal of Narjing Medical University,Nanjing 210029,China)
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第4期286-288,共3页
Chinese Journal of Arteriosclerosis
基金
江苏省教育厅自然科学基金资助项目(JB32006)
