摘要
目的探讨5种常用抗肿瘤药长春新碱、阿霉素、平阳霉素、顺铂、环磷酰胺对KBV200耐药细胞株的增殖抑制作用及苦参碱的耐药逆转作用。方法以喉癌KBV200耐药细胞株为研究对象,采用MTT法观察5种抗肿瘤药物对其增殖抑制作用;并采用MTT法观察苦参碱对KBV200耐药细胞株的耐药逆转作用。结果长春新碱、平阳霉素、环磷酰胺对KBV200耐药细胞株的增殖抑制作用差,而阿霉素和顺铂对KBV200耐药细胞株的抑制作用好;当苦参碱浓度大于200μg/m l时对KBV200耐药细胞株具有直接抑制作用;苦参碱逆转KBV200耐药细胞株对长春新碱、阿霉素耐药的作用较强,逆转KBV200耐药细胞株对平阳霉素、顺铂、环磷酰胺的耐药作用较弱。结论KBV200耐药细胞株对长春新碱、平阳霉素、环磷酰胺3种药物有不同程度耐药;苦参碱能逆转KBV200耐药细胞株对长春新碱、阿霉素的耐药。
Objective To observe the inhibitory effect of 5 chemotherapeutic agents including vincristine, adriamycin, pingyangmycin, cisplatin, cyclophosphamide on the KBV200 cell line and to study the reverse effect on the muhidrug resistance of Matrine. Methocls MTT assay was used to investigate the inhibition ability of 5 drugs on the cell line and the reverse effect on the muhidrug resistance of Matrine. Results Vincristine, pingyangmycin, cyclophosphamide had less inhibitory effects than adriamycin, cisplatin on the KBV200 cell line. Matrine had the ability to reverse the muhidrug resistance of the cell line to vincristine, adriamycin. Conclusion KBV200 cell line is multidrug-resistant to vincristine, pingyangmycin, cyclophosphamide. Matrine can enhance the sensitivity of KBV200 to the vincristine,
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第11期1231-1233,共3页
Journal of Third Military Medical University
关键词
抗肿瘤药物
KBV200细胞株
耐药逆转
苦参碱
adriamycin.chemotherapeutic agent
KBV200 multidrug resistance cell line
reversion of multidrug resistance
Matrine
