摘要
辣根过氧化物酶（ＨＲＰ）免疫中华鳖后，刺激其脾脏的组织结构发生变化。免疫后第五天，动脉周围淋巴鞘（ＰＡＬＳ）内嗜派若宁细胞增多，主要为淋巴母细胞，椭球周围淋巴鞘（ＰＥＬＳ）和红髓则未见变化。免疫后第十天，白髓增殖显著，嗜派若宁细胞增多到整个免疫过程中的最高峰，尤其是ＰＥＬＳ内嗜派若宁细胞数量几乎与ＰＡＬＳ持平。免疫后第十五天，白髓比例明显减少，可见许多处于不同排空程度的淋巴鞘，嗜派若宁细胞也开始减少，ＰＥＬＳ尤为显著，浆细胞增加。免疫后第二十一天，白髓恢复正常，但ＰＡＬＳ附近红髓中装细胞增加明显。免疫后第二十九天，脾脏的结构趋于正常。整个免疫过程中，未见到淋巴小结和生发中心。
Changes of the structure of the spleen in the Amyda sinensis immunized with HRP (horeseradish peroxidase ) were observed. By day 5 post - immunization, the increase of the phroninophilic cell, which was mainly lymphoblast, was apparent in the periarteriolar sheath (PALS), but not in the periellipsoidal sheath (PELS) and the red pulp. By day 10, the white pulp proliferated markedly, the number of pyroninophilic cells maxmized during the whole immunization, particularly in the PELS, pyroninophilic cells were nearly equal to those in the PALS. By day 15, the white pulp reduced markedly, the large number of lymphoid sheaths in various stages of emptiness were obsevered, the amount of pyroninophilic cells started to fall, particularly obvious in the PELS. Plasma cells increased. By day 21, the amount of plasma cells rised apparently in the red pulp near the PALS. By day 29, the structure of the spleen of Amyda sinensis tended to normal level. Lymph nodules and germinal centers were not found during the whole immunization.
出处
《中国水产科学》
CAS
CSCD
1996年第1期21-26,共6页
Journal of Fishery Sciences of China
关键词
中华鳖
脾脏
形态结构
Ampda sinensis, Spleen, Horeseradish peroxidase (HRP), Pyroninophilic cell
