摘要
目的观察香菇多糖联合顺铂对Lewis荷瘤小鼠肿瘤细胞凋亡的影响。方法选用小鼠Lewis肺癌肿瘤细胞株(1×107)皮下注射接种,制作肿瘤动物模型后,随机分为正常对照组、肿瘤对照组、香菇多糖组、顺铂组、香菇多糖加顺铂组,进行光、电镜形态学观察。结果形态学观察治疗组与对照组比较,肿瘤团块较小,异型性不明显,病理性核分裂和间质血管较少,淋巴细胞浸润明显。电镜下均可见凋亡小体形成,其中以香菇多糖加顺铂组最明显。结论香菇多糖具有协同顺铂抗肺癌作用,其作用机制是通过诱导肿瘤细胞凋亡及刺激淋巴细胞增生的作用而实现的。
Objective To observe the effects of Lentinan（LNT） combined with Cisplatin （DDP） on the apoptosis induction in mice bearing Lewis lung carcinomas （LLC）. Methods Lewis lung carcinoma ceils （1 × 10^7） were subcutaneously inoculated into BALB/c mice. Mice were randomized into control groups and treatment groups. Observe the morphologic views under light microscope （LM） and electron microscope （EM）. Results Compared with the control groups, the treatment groups were morphologic characteristic by relatively smaller in size, cell atypia were not evident, pathalogical karyokinesis were less interstitial blood vessel were fewer, while lymphocyte infiltrate into the interstitial. Apoptotic bodies could be seen under EM. Within all treatment groups, LNT＋DDP group showed significant difference. Conclusions Lentinan combined with Cisplatin showed significant antitumor effects, and mechanism maybe inducing the tumor cell apoptosis and stimulate the proliferation of lymphocyte.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第6期23-24,共2页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
香菇多糖
顺铂
肿瘤细胞凋亡
电镜
Lentinan
Cisplatin
tumorcell apoptosis
electron microscope
