摘要
采用改进的聚丙烯酰胺凝胶电泳对5种黑稻主要栽培品种进行了EST同工酶谱分析。结果表明,此方法分辨率高。测试各品种的EST同工酶存在丰富的多态性,其电泳图谱具有鲜明的特征,并构成了各自的“指纹”。其中黑优粘和云黑酶谱条带数多达15条,a1和a3为5个品种的共有谱带。通过EST同工酶谱相似度指数分析,黑优粘与黑丰糯、乌贡1号相似度指数大于0.75,说明这3个品种间亲缘关系较近。
Analysis of the Esterase isozyme of black rices variety was carried out by PAGE, The results showed that the Esterase isozyme had a plenty of polymorphism on account of high resolution. Each variety of black rices has it＇ s own unique band pattern. Compared with their finger prints, the results showed their bands reach 15 at most. a1 and a3 bands are common for five varieties. Analysis of similarity degree index of EST Isozyme indicated that three varieties had a near relationship becanse of a high index which is above 0.75.
出处
《种子》
CSCD
北大核心
2006年第4期21-22,26,共3页
Seed
基金
陕西省重点实验室访问学者科研计划项目(02JF04)
关键词
黑稻
EST同工酶谱
纯度检测
亲缘关系
Black rice Esterase isozyme Relationship Purity identification
