摘要
目的研究基质金属蛋白酶-7(MMP-7)、基质金属蛋白酶抑制因子-1(TIMP-1)在子宫腺肌病中的表达,探讨二者之间的相关性及其在子宫腺肌病发病机制中的作用。方法采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法,检测78例子宫腺肌病患者的原位内膜、异位内膜及80例正常子宫内膜中MMP-7和TIMP-1表达情况。结果①MMP-7在子宫腺肌病原位内膜、异位内膜中的表达显著高于正常子宫内膜,差异有显著意义(P<0．05);②TIMP-1在正常子宫内膜的表达高于腺肌病的原位内膜、异位内膜,差异有显著意义(P<0．05)。③在正常子宫内膜组织中MMP-7与TIMP-1之间具有负相关关系,在腺肌病的原位内膜、异位内膜中二者不具有相关性。结论 MMP-7表达增强和MMP-7/TIMP-1 的比例失调,是子宫腺肌病的原位内膜及异位内膜细胞具有侵袭能力的原因之一,从而为治疗本病提供了循证医学依据。
Objective To observe the expression of matrix metalloproteinase-7 （MMP-7） and tissue inhibitors of matrix metalloproteinase-1 （TIMP-1） in adenomyosis. To explore the correlation between the expression of MMP-7 and TIMP-1 in adenomyosis, and to study the possible role of MMP-7 and TIMP 1 in the pathogenesis of adenomyosis. Methods The expression of MMPT7 and TIMP-1 in 78 ectopic and eutopic endometrium in adenomyosis were detected by immunohistochemical methods in comparison with 80 normal endometrium. Results ①the expression levels of MMP 7 in ectopie and eutopic of adenomyosis tissues were significantly higher than those in the normal endometrium（P〈0.05）. ②The expression levels of TIMP-1 on ectopic and eutopic endometrium of adenomyosis were controlled with normal endometrium, P〈0. 05, having statistical significance. ③there was significantly negative correlation between expression of MMP-7 and TIMP-1 in normal endometrium, but there was no correlation in ectopic and eutopic endometrium of adenomyosis. Conclusion The high expression of MMP-7 and the lopside development on MMP-7/TIMP-1 ratio may participate in the process of invasion and tissue modeling that is hypothesized to occur in the pathogenesis of adenomyosis.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2006年第3期134-136,共3页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
广东省医学科研基金(编号:A2002565)
