摘要
目的:探讨二硫化碳(CS2)对大鼠海马一氧化氮合酶(NOS)活力和基因表达的影响。方法:以吸入染毒法制作不同浓度CS2中毒大鼠模型:染毒2个月后,用Morris水迷宫法检测实验大鼠的学习记忆功能;以NOS测定试剂盒测定大鼠海马NOS活力;以半定量逆转录一聚合酶链式反应(RT-PCR)法测定神经元型一氧化氮合酶(nNOS)mRNA 含量的变化。结果:Morris水迷宫测试显示染毒后大鼠平均逃逸潜伏期较对照组延长,差异有显著性;各CS2染毒组大鼠海马NOS活性降低,与对照组比较差异有显著性,随CS2浓度的增加NOS活性降低,各染毒组间比较差异有显著性差异;染毒后海马nNOS mRNA含量比对照组显著减少,与CS2浓度呈剂量依赖关系,差异有显著性意义。结论:CS2致大鼠海马NOS活力降低及nNOS mRNA含量减少可能是CS2干扰学习记忆功能的机制之一。
Objective： To explore the effect of carbon bisulfide（CS2 ） on the activity and gene expression of nitricoxide synthase（NOS） in rat hippocampus. Methods： The toxic rat models were made by inhaling various doses CS2. After 2 months of inhaling intoxication, the learning and memory ability of all groups were examined by Morris Water Maze, the activity of NOS in hippocampus was detected by NOS Test Kit and Neuronal NOS（nNOS） mRNA content was measured by semi quantitative reverse transcription polymerase chain reaction（RT PCR） method. Results： Compared with the controls, Morris Water Maze showed that the average run away latent period of CS2-poisoned groups elongated and the activity of NOS significantly increased. With the increase of CS2 concentration, the activity of NOS decreased. CS2 treatment significantly decreased nNOS mRNA content, which was CS2 concentration dependent. Conclusion： These results suggest that the activity of NOS and the expression of nNOS might be related to the effect of CS2 on learning and memory.
出处
《脑与神经疾病杂志》
2006年第3期199-202,共4页
Journal of Brain and Nervous Diseases
基金
国家自然科学基金资助项目(No.30471442)
