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兔急性脑缺血再灌注扩散加权成像的实验研究 被引量：2

An experimental study on acute cerebral ischemia and reperfusion with magnetic resonance diffusion weighted imaging of rabbits
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摘要 目的探讨兔脑大脑中动脉缺血再灌注(MCAO I/R)后磁共振扩散加权成像(DW I)的特点。方法将制作成功的兔MCAO I/R模型58只,随机分为永久性缺血组30只(再分为缺血1、3、6、12、24、48 h组,每组5只)和缺血再灌注组28只(再分为缺血再灌注0、2、5、11、23、47 h组,每组分别为5、5、4、5、4只);另取10只动物行假手术分别作为缺血组(5只)及再灌注组(5只)的对照组;观察不同时间DW I像上高信号区范围变化及表观扩散系数(ADC)的演变特点。结果各组于缺血1 h均见到DW I像上明显的高信号,并伴ADC值的下降。缺血组不同缺血时间点各组平均ADC值呈先下降后上升的趋势。与缺血1 h比,各缺血时间点DW I像上的高信号区范围均有不同程度的增大,24 h后趋于稳定。再灌注组与再灌注前(缺血1 h)相比,再灌注2、5 h组均表现为DW I像上高信号区范围的缩小及ADC值升高;再灌注11 h组表现为高信号范围的增大并ADC值升高;再灌注23、47 h组表现为高信号范围的增大而ADC值出现较明显的下降。结论急性脑缺血后DW I像高信号区及ADC值的下降经早期再灌注后可明显改善,但持续的再灌注可能导致ADC值的再次下降。 Objective To discuss the changes of magnetic resonance diffusion weighted imaging（DWI） in acute middle cerebral artery occlusion ischemia and reperfusion（MCAOI/R）in rabbits. Methods 58 successful MCAO models were randomly divided into permanent ischemic group （n = 30） which were further averagely subdivided into ischemic 1, 3, 6, 12, 24, 48 h group and ischemic reperfusion group （ n = 28） which were divided into reperfusion 0, 2, 5, 11,23,47 h group consisting respectively 5, 5, 5, 4, 5,4 cases. Another 10 rabbits were averagely divided into ischemic contrast and reperfusion contrast. The changes of hyperintensity area on DWI and apparent diffusion coefficient （ADC） were measured respectively in different groups. Results All rabbits showed hyperintensity area on DWI with declined ADC following ischemic 1 h. The mean ADC of each time point in ischemic group declined at first and then gradually increased. Compared with ischemic 1 h, the hyperintensity area on DWI in other time points was obviously enlarged and became steadily after 24 h in ischenile group. Compared with ischemic 1 h, hyperintensity area decreased in reperfusion 2, 5 h groups, increased in reperfusion 11,23 and 47 h groups; ADC increased in reperfusion 2, 5 and 11 h groups and decreased in reperfusion 23 and 47 h groups. Conclusions The hyperintensity area and the decreased ADC in acute cerebral ischemia could be improved by early reperfusion, but continuous reperfusion could induce the secondary decreased ADC.
作者 刘学军 金丽英 孔令琦 刘广义 隋庆兰 杨学伟
机构地区 青岛大学医学院附属医院影像中心 青岛大学附属医学院脑血管病研究所
出处 《中国老年学杂志》 CAS CSCD 北大核心 2006年第5期641-644,共4页 Chinese Journal of Gerontology
基金 山东省卫生厅和青岛市科技局基金资助项目(04-2-NY-46)
关键词 脑缺血 再灌注 磁共振成像 扩散加权成像 表观扩散系数 Cerebral ischemia Reperfusion Magnetic resonance imaging Diffusion weighted imaging Apparent diffusion coefficient
分类号 R445.2 [医药卫生—影像医学与核医学] R332 [医药卫生—人体生理学]
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参考文献12

  • 1Molnar L,Hegedus K,Fekete I.A new model for inducing transient cerebral ischaemia and subsequent reperfusion in rabbits without craniectomy[J].Stroke,1988:19 (10):1262-6.
  • 2孔令琦,谢敬霞,韩鸿宾,刘洪东,郎宁,裴新龙.提高线栓法大脑中动脉闭塞性兔脑缺血模型稳定性和可重复性的研究[J].中国医学影像技术,2004,20(2):209-212. 被引量：29
  • 3Rana AK,Wardlaw JM,Armitage PA,et al.Apparent diffusion coefficient (ADC) measurements may be more reliable and reproducible than lesion volume on diffusion weighted images from patients with acute ischaemic stroke-implications for study design[ J ].Magn Reson Imaging,2003;21 (6):617-24.
  • 4Fiehler J,Knudsen K,Kucinski T,et al.Predictors of apparent diffusion coefficient normalization in stroke patients[ J ].Stroke,2004; 35 (2):514-9.
  • 5殷信道,顾建平,王丽萍,卢铃铨,冯晓源.超急性脑梗死再灌注弥散加权——灌注磁共振成像实验研究[J].实用放射学杂志,2004,20(9):769-773. 被引量：8
  • 6Beaulieu C,de Crespigny A,Tong DC,et al.Longitudinal magnetic resonance imaging study of perfusion and diffusion in stroke:evolution of lesion volume and correlation with clinical outcome[ J ].Ann Neurol,1999;46(4):568-78.
  • 7Dardzinski BJ,Sotak CH,Fisher M,et al.Apparent diffusion coefficient mapping of experimental focal cerebral ischemia using diffusion-weighted echo-planar imaging[J].Magn Reson Med,1993 ;30(3):318-25.
  • 8Kazemi M,Silva MD,Li F,et al.Investigation of techniques to quantify in vivo lesion volume based on comparison of water apparent diffusion coefficient (ADC) maps with histology in focal cerebral ischemia of rats[J].Magn Reson Imaging,2004 ;22 (5):653-9.
  • 9Laszlo Olah,Stefan Wecker,et al.Secondary deterioration of apparent diffusion coefficient after 1-hour transient focal cerebral ischemia in rats[J].J Cereb Blood Flow Metab,2000 ;20:1474-82.
  • 10Provenzale JM,Soreencen AG.Diffusion-weighted MR imaging in acute stroke:theoretic considerations and clinical applications[J].AJR,1999;173(6):1459-67.

二级参考文献39

  • 1Fiskum G, Murphy AN, Beal MF. Mitochondria in neurodegeneration: acute ischemia and chronic neurodegenerative diseases[J].J Cereb Blood Flow Metab,1999,19(4):351-369.
  • 2Guo J,Meng F,Zhang G,et al.Free radicals are involved in continuous activation of nonreceptor tyrosine protein kinase c-Src after ischemia/reperfusion in rat hippocampus[J].Neurosci Lett,2003,345(2):101-104.
  • 3Charriaut-Marlangue C, Margaill I, Represa A, et al. Apoptosis and necrosis after reversible focal ischemia: an in situ DNA fragmentation analysis[J].J Cereb Blood Flow Metab,1996,16(2):186-194.
  • 4Abe K, Aoki M, Kawagoe J, et al. Ischemic delayed neuronal death: a mitochondrial hypothesis[J].Stroke,1995,26(8):1478-1489.
  • 5van Dorsten FA, Hata R, Maeda K, et al. Diffusion-and perfusion -weighted imaging of transient focal cerebral ischemia in mice[J].NMR Biomed,1999,12(8):525-534.
  • 6Jiang Q,Zhang RL,Zhang ZG, et al. Magnetic resonance imaging indexes of the therapeutic efficacy of recombinant tissue plasminogen activator treatment of rat 1 and 4 hours after embolic stroke[J].J Cereb Blood Flow Metab,2000,20(1):21-27.
  • 7Sakoh M, Rohl L, Gyldensted C, et al. Cerebral blood flou and blood volume measured by magnetic resonance imaging bolus tracking after acute stroke in pigs: comparison with [15O]H2O positron emission tomography[J].Stroke,2000,31(8):1958-1964.
  • 8Li F, Silva MD, Sotak CH, et al. Temporal evolution of ischemic injury evaluated with diffusion-, perfusion- and T2-weighted MRI[J].Neurology,2000,54(3):689-696.
  • 9Li F, Han SS, Tatlisumak T, et al. Reversal of acute apparent diffusion coefficient abnormalities and delayed neuronal death following transient focal brain ischemia in rats[J].Ann Neurol,1999,46(3):333-342.
  • 10Muller TB, Haraldseth O, Jones RA, et al. Combined perfusion and diffusion -weighted magnetic resonance imaging in a rat model of reversible middle cerebral artery occlusion[J].Stroke,1995, 26(3):451-458.

共引文献100

同被引文献21

  • 1杨冀萍,刘怀军,刘瑞春,李春岩,张晖,池琛,王志红,李林芳.兔局灶性脑缺血再灌注模型的建立和影像学评价[J].脑与神经疾病杂志,2006,14(5):354-356. 被引量：9
  • 2Li F, Silva MD, Sotak CH, et al. Temporal evolution of ischemic injuly evaluated with diffusion-, perfusion- and T2-weighted MRI. Neurology. 2000, 54:689-696.
  • 3Meng X, Fisher M, Shen Q, et al. Characterizing the diffusion/ perfusion mismatch in experimental focal cerebral ischemia. Ann Neurol. 2004, 55 : 207-212.
  • 4Rojas S, Martin A, Justicia C, et al. Modest MRI signal intensity changes precede delayed cortical necrosis after transient focal ischemia in the rat. Stroke. 2006, 37:1525-1532.
  • 5Garcia JH, Liu K-F, Ho K-L. Neuronal necrosis after middle cerebral artery occlusion in Wistar rats progresses at different time intervals in lhe caudoputamen and the cortex. Stroke. 1995, 26:636-643 .
  • 6Garcia JH, Liu K-F, Ye Z-R, et al. Incomplete infarct and delayed neuronal death after transient middle cerebral artery occlusion in rats. Stroke, 1997, 28:2303-2310.
  • 7Restrepo L, Jacobs MA, Barker PB,et al. Assessment of transient ischemic attack with diffusion- and perfusion-weighted imaging. AJNR Am J Neuroradiot. 2004, 25 : 1645-1652.
  • 8Sicard KM, Henninger N, Fisher M, et al. Long - term changes of functional MRI - based brain function , behavioral status , and histopathology after transient focal cerebral ischemia in rats. Stroke. 2006, 37:2593-2600.
  • 9Inatomi Y, Kimura K, Yonehara T, et al. DWI abnormalities and clinical characteristics in TIA patients. Neurology. 2004, 62: 376- 380.
  • 10Garcia JH, Lassen NA, Weiller C, et al. Ischemic stroke and incomplete infarction. Stroke. 1996, 27:761-765.

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中国老年学杂志
2006年 第5期

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