摘要
目的从分子水平及骨代谢角度探讨健骨颗粒对成骨细胞功能的调控作用以及对骨吸收的影响。方法通过切除大鼠卵巢建立骨质疏松模型,药物干预,分别运用酶联免疫吸附法和原位杂交等手段,检测模型鼠尿脱氧吡啶酚(D-Pyr)水平和骨组织整合素β1(Itgβ1)mRNA表达。结果切除卵巢后模型鼠尿D-Pyr水平明显升高(P<0·01),骨组织成骨细胞Itgβ1mRNA表达降低。健骨颗粒治疗12周,骨质疏松模型鼠尿D-Pyr水平明显下降(P<0·05),而骨组织成骨细胞数量以及Itgβ1mRNA表达均显著增加。结论健骨颗粒通过降低骨质疏松模型鼠骨基质中Ⅰ型胶原纤维的分解代谢,提高成骨细胞的成骨活性,从而改善或扭转因E2下降出现的成骨不足病理态势,发挥“健骨”之功。
Objective To investigate the regulatory effect of Jiangu Granule （JGG） on osteoblast and its effect on bone absorption on the molecular level and from aspect of bone metabolism. Methods Osteoporosis model SD rats established by ovariectomy were interfered by drug. Levels of urinary deoxypyridinoline （D-Pyr） and osseous integrin β1 （Itgβ1） mRNA expression were detected by ELISA and in situ hybridization, respectively. Results Level of urinary D-Pyr increased （P 〈 0.01 ） and osseous Itgβ1 mRNA expression decreased significantly in the model rats. After treatment with JGG for 12 weeks, the D-Pyr levels in them decreased significantly （P〈0.05）, while the quantity of osteoblasts and the expression of Itgβ1 mRNA in bone tissue increased obviously. Conclusion JGG can not only decrease the catabolism of collagen fiber type I in bone matrix, but also promote the osteogenic activity of osteoblast. So, it can improve or reverse the pathological tendency of osteogenesis shortage caused by E2 reduction to play its action in strengthening bone.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2006年第5期435-438,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
福建省自然科学基金项目(No.C0410031)
福建省科技三项费用项目(No.K02083)
