摘要
目的:观察全反式维甲酸(ATRA)对人胰腺癌细胞株PC-3生长的抑制作用以及细胞株PC-3 Survivin基因表达的变化。方法:应用MTT比色法检测细胞增殖抑制率,RT-PCR法测定Survivin基因表达,流式细胞仪检测细胞凋亡。结果:经过ATRA作用后,PC-3细胞增殖受到抑制,呈时间和剂量依赖性。凋亡比例明显增加,Survivin mRNA的表达随着作用时间的延长而呈逐渐下降趋势。结论:ATRA对人胰腺癌细胞株PC-3的生长有抑制作用,同时可诱导其凋亡,提示其可能与Survivin基因表达下调有关。
Objective： To observe the inhibitiory effects of all-trans retinoie acid （ATRA） on the pancreatic cancer cells growth in vitro and Survivin gene expression induced by ATRA, Methods： The inhibition rates of proliferation were detected by MTT assay. RT-PCR and flow cytometry were used to detect the expression of Survivin gene and the apoptotic rates. Results： After the treatment with ATRA, the proliferation of PC-3 was inhibited in dose-dependent and time-dependent manner,and the apoptotic rates of PC-3 cells were increased significantly. The expression of Survivin mRNA was decreased obviously in PC-3 cells receiving ATRA treatment compared with those receiving no treatment. Conclusion： ATRA can inhibit pancreatic cancer cells growth,induce cells apoptosis. Survivin gene probably contributes to the apoptosis of pancreatic cancer cells induced by ATRA.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第6期389-392,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅135重点学科基金资助项目(苏卫科教[2001]31号)
