摘要
Objective：To study the effect of μ-opioid receptor （MOR） expression in the inflammatory skin tissue and the effect of electroacupuncture （EA） on the topical immunoreaction （IR） of MOR positive fibers in adjuvant arthritic （AA） rats. Methods： A total of 48 SD rats were randomized into control （n = 8）, model （n = 10）, focus-side-EA （n = 10）, non-acupoint-EA （n = 10）, and healthy-side-EA （ n = 10） groups. AA model was established by subcutaneous injection of complete Freund＇s adjuvant （CFA, 50 μL） into the left hind paw. EA （4-16 Hz, 0.5-1.5 V） was applied to “Huantiao” （环跳 GB 30） and“Yanglingquan” （阳陵泉 GB 34） on the focus or healthy side and non-acupoints for 30 min. Non-acupoints used were the two sites 5 mm to GB 30 and GB 34 on the healthy side. The topical MOR IR-positive fibers in the dermal and subcutaneous tissues of the focus was stained with immunohistochemical method. The severity of pain was detected by foot （anklejoint）-bending test. Results： Compared with model group, the “foot-bending test” score decreased significantly in focus-side-EA group on the 9^th and 11^th day （P〈 0.05） and in non-acupoint-EA group on the 8^th, 9^th and 11thd after injection of CFA （P 〈 0.05）, indicating that EA of bilateral GB 30 and GB 34 and non-acupoints all can relieve pain. From the 13^th day on, no significant differences were found in “foot-bending test” scores among the 3 EA groups and model group （P 〉0.05）. In comparison with control group, the area values of MOR IR-positive nerve fibers in the focus tissue were significantly higher in 3 EA groups （P 〈 0.05）. The area values of MOR IR-positive nerve fibers in the focus in model group and 3 EA groups were significant higher than that in control group （P〈 0.05）. Compared with model group, the area values of MOR IR-positive fibers in focus-side-EA group and healthy-side-EA group increased significantly （P 〈 0.05）; while those of MOR IR-positive fibers in non-acupoint-EA group and healthy-side-EA group were significantly lower than that in focus-side-EA group （ P 〈 0.05 ）, and no significant differences were found among model group, healthy-side-EA group and non-acupoint-EA group in the area of MOR IR-positive fibers （P 〉0.05）, indicating a stronger effect of EA of acupoints on the focus side. Conclusion： EA of GB 30 and GB 34 can relieve inflammatory pain and up-regulate the expression of MOR IR-positive fibers in the focal skin tissues in AA rats, exerting anti-inflammatory and analgesic effects.
客观：学习 -opioid 的效果在煽动性的皮肤织物和 electroacupuncture (EA ) 的效果的受体(粗腐殖质) 表示在在助手的粗腐殖质积极纤维的热门免疫反应(红外) 关节炎(AA ) 老鼠。方法：48 只 SD 老鼠的一个总数被使随机化进控制(n =8 ) ，模型(n= 10 ) ， focus-side-EA (n = 10 ) ， non-acupoint-EA (n = 10 ) ，并且 healthy-side-EA (n = 10 ) 组。 AA 模型被完全的 Freund 的助手的下的注射建立( CFA ， 50 L )进左后部的 paw.EA ( 4-16 Hz ， 0.5-1.5 V )为 30 min 在焦点或健康方面和 non-acupoints 上被用于“ Huantiao ”( GB 30 )和“ Yanglingquan ”( GB 34 )。使用的 Non-acupoints 是二个地点 5 公里到健康方面上的 GB 30 和 GB 34。在焦点的真皮、下的纸巾的热门粗腐殖质红外积极的纤维与 immunohistochemical 方法被染色。疼痛的严厉被把测试弄弯的脚(anklejoint ) 检测。结果：与模型组相比，“弯曲脚的测试” 20 在 第9 和 第11 天( P0.05 )并且在 non-acupoint-EA 组在 focus-side-EA 组显著地减少了在上 第8 ， 第9 并且在 CFA ( P 0.05 )的注射以后的 11thd ，都显示双边的 GB 30 和 GB 34 和 non-acupoints 的那 EA 能减轻疼痛。从第 13 天在上，没有重要差别在 3 个 EA 组和模型组(P0.05 ) 之中在“弯曲脚的测试”被发现分数。与控制组比较，在焦点织物的粗腐殖质红外积极的神经纤维的区域价值在在在模型的焦点的粗腐殖质红外积极的神经纤维的 .The 区域价值组织的 3 个 EA 组( P0.05 )是显著地更高的， 3 个 EA 组在控制组( P0.05 )比那高重要。与模型组相比，区域在组和 healthy-side-EA 组显著地增加了的 focus-side-EA (P 0.05 ) 粗腐殖质红外积极的纤维珍视；当在 non-acupoint-EA 组和 healthy-side-EA 组的粗腐殖质红外积极的纤维的那些在 focus-side-EA 是比那显著地低的时，组( P 0.05 )，和没有重要差别在粗腐殖质红外积极的纤维( P0.05 )的区域在模型组， healthy-side-EA 组和 non-acupoint-EA 组之中被发现，在焦点方面上显示 acupoints 的 EA 的更强壮的效果。结论：GB 30 和 GB 34 的 EA 能减轻煽动性的疼痛和起来调整在在 AA 老鼠的焦点的皮纸巾的粗腐殖质红外积极的纤维的表示，施加反煽动性、止痛的效果。
基金
This study is subsidized by Chinese National Natural Science Foundation [Key Research Project (90209009) ]Hubei Natural Science Foundation (2002AB124)Foundation for Scientific Research of Huazhong University of Science and Technology
