摘要
目的建立一种同时快速测定大鼠纹状体中谷氨酸（Glu）、天冬氨酸（Asp）、γ-氨基丁酸（GABA）、甘氨酸（Gly）、牛磺酸（Tau）5种氨基酸类神经递质的高效液相色谱法。方法采用Kromasil C18（4．6×250mm，5μm）为色谱柱，丹酰氯为柱前衍生试剂，以曲唑酮为内标，以0．1mol／L醋酸钠缓冲液：甲醇=58：42、14mmol／L庚烷磺酸钠为流动相等度洗脱。结果在1—200mg／L范围内，各组分线性关系良好（r=0．999）。各氨基酸的平均回收率为79．1%-92．9％。结论本方法简便、快速、准确，可用于临床大样本测定和研究神经及精神疾病氨基酸类神经递质的改变。
Objective To develop a high performance liquid chromatography （HPLC） for simultaneous separation and rapid determination of five amino acid [ glutamate（Glu）, aspartate（Asp）, γ-aminobutyric acid（GABA） , glycine （Gly）, taurine（Tau）] neurotransmitters in the striatum of rat brain. Methods The HPLC column was Kromasil CIs （4.6 × 250 mm, particle size 5 μm）. The precolumn derivatization reagent was 1-dimethyl aminonaphthalenesulfonyl chloide and trazodone was used as internal standard. The mobile phase was a mixture of 42% methanol and 58% 0. 1 mol/L sodium acetate （V/V） ,which involved 1% tetrahydrofuran, 14 mmol/L 1-heptanesulfonic acid sodium salt. Isocratic elution was used. Results The linear range of the assay was 1 - 200 mg/L. The calibrations were characterized by a coefficient of correlation higher than 0.999. The average recoveries of amino acid were from 79.1% to 92.9%. Conclusions The method is simple, rapid and accurate. It is useful for studying changes of neurotransmitter amino acid levels in neuropsychiatric disorders.
出处
《检验医学》
CAS
北大核心
2006年第3期215-218,共4页
Laboratory Medicine
基金
江苏省自然科学基金资助项目(BK2001007)
关键词
氨基酸
神经递质
高效液相色谱
Amino acid
Neurotransmitter
High performance liquid chromatography
