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Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phase High Performance Liquid Chromatography Under Salt and Alkali Stress 被引量:7

Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phase High Performance Liquid Chromatography Under Salt and Alkali Stress
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摘要 Several organic acids accumulated in Kochia Scoparia shoots and roots were studied by means of reverse-phase high performance liquid chromatography with a C18 column. Five types of binary organic acids were separated. The organic acid concentrations were determined in K. Scoparia seedlings stressed by saline (NaCI) and alkaline (NaHCO3) at the same Na^+ concentration. Concentrations of organic acids are stimulated by alkaline because the cells will adjust their pH values through the accumulation of organic acids, when the environment is basic. The concentrations of oxalic acid and succinic acid are higher than those of other organic acids, including tartaric acid and malic acid, and the concentration of citric acid is the lowest. The concentrations of the organic acids in the roots are higher than those in the shoots under salt(NaCI) stress, but the results are opposite while the roots are under alkali ( NaHCO3 ) stress. This indicates that there are different adaptive strategies for K. Scopar/a seedlings in organic acid metabolism under salt and alkali stress. Several organic acids accumulated in Kochia Scoparia shoots and roots were studied by means of reverse-phase high performance liquid chromatography with a C18 column. Five types of binary organic acids were separated. The organic acid concentrations were determined in K. Scoparia seedlings stressed by saline (NaCI) and alkaline (NaHCO3) at the same Na^+ concentration. Concentrations of organic acids are stimulated by alkaline because the cells will adjust their pH values through the accumulation of organic acids, when the environment is basic. The concentrations of oxalic acid and succinic acid are higher than those of other organic acids, including tartaric acid and malic acid, and the concentration of citric acid is the lowest. The concentrations of the organic acids in the roots are higher than those in the shoots under salt(NaCI) stress, but the results are opposite while the roots are under alkali ( NaHCO3 ) stress. This indicates that there are different adaptive strategies for K. Scopar/a seedlings in organic acid metabolism under salt and alkali stress.
出处 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2006年第3期315-318,共4页 高等学校化学研究(英文版)
关键词 Kochia Scoparia(L. Sehrad Salt stress Alkaline stress Organic acid HPLC Kochia Scoparia(L. ) Sehrad Salt stress Alkaline stress Organic acid HPLC
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