摘要
以不同时期的花蕾为外植体,研究了影响丛生芽诱导、增殖、壮苗生根的主要因素.结果表明：处于原芽期的花蕾是诱导丛生芽的最佳时期;适合诱导丛芽的培养基是MS＋BA 0.1～2.0 mg/L＋2.4-D 0.2～1.0 mg/L＋GA 0.5 mg/L＋LH 500 mg/L＋3%蔗糖,适合丛生芽增殖成苗的培养基是MS＋6-BA 1.0～1.5 mg/L＋2.4-D 0.2～0.5 mg/L＋3%蔗糖,适合壮苗生根的培养基是1/4MS＋IBA 0.1 mg/L＋1%蔗糖.愈伤分化过程中的生理变化：可溶性糖与可溶性蛋白的含量有着相同的变化趋势,先增加再减少,然后再增加.叶绿素含量则呈上升趋势,这说明叶绿素的适量形成有利于分化.
The main factors of crowd shoot induction, propagation and rooting of Hemerocailis fulva（L） were studied by using different growth period of bud. The results showed that the primary bud is better explants than others. The suitable medium for crowd shoot induction is MS ＋ BA 0.1 - 2.0 mg/L ＋ 2.4 - D 0.2 - 1.0 mg/L ＋ GA 0.5 mg/L ＋ LH 500 mg/L ＋ 3%sucrose. The best medium for shoot propagation is MS＋6- BA 1.0- 1.5 mg/L ＋ 2.4- D 0.2 - 0.5 mg/L ＋ 3 % sucrose. The proper medium for rooting is 1/4MS ＋ IBA0. lmg/L ＋ 1% sucrose. The physiological change were studied in callus during the differentiation： The condition of soluble sugar and soluble protein had the same trend, up - down - up. The condition of chlorophyll was upward trend, which might have some thing with differentiation.
出处
《大连民族学院学报》
CAS
2006年第3期44-46,共3页
Journal of Dalian Nationalities University
关键词
欧洲矮化大花萱草
组织培养
生理变化
Hemerocallis fulva（L）
tissue culture
physiological change
