摘要
目的观察氯胺酮对小鼠避暗潜伏期及错误次数的影响,并初步探讨其机制。方法按分层随机区组设计将小鼠分为6组(n=10):生理盐水组(NS组),一叶秋碱(1 mg.kg-1)组(S组),氯胺酮(5 mg.kg-1、10 mg.kg-12、0 mg.kg-1)组(K5、K10、K20组),一叶秋碱(1 mg.kg-1)+氯胺酮(20 mg.kg-1)组(SK组)。用避暗实验测定给药后24 h小鼠的避暗潜伏期及错误次数。结果与NS组相比,K10、K20组的避暗潜伏期缩短(P均<0.01)、错误次数增多(P<0.05,P<0.01),似呈剂量依赖性。与K20组相比,SK组的避暗潜伏期延长(P<0.01)、错误次数减少(P<0.01),但不能恢复至NS组水平(P<0.01,P<0.05)。结论GABAA受体部分介导了氯胺酮对小鼠的记忆损害作用。
Objective To investigate the effect of ketamine on step- through latency and number of errors and to study the primary mechanism. Methods The mice were randomly divided into six groups （ n = 10 each） ： normal saline group（NS group）, securinine 1mg·kg^-1 group（S group）, ketamine 5 mg·kg^-1, 10 mg·kg^-1 ,20 mg·kg^-1 groups（ K5, K10, K20 groups）, securinine 1mg·kg^-1 ＋ ketamine 20 mg·kg^-1 group（SK group）. The step - through latency and number of errors were recorded 24 h after the drugs were given in the step - through task. Results In the ketamine groups, the step - through latency decreased and the number of errors increased（P 〈0.05, P 〈0.01）, seemingly in a dose- dependent manner. As the SK group was compared with the K20 group, the step-through latency was increased and the number of errors decreased（ P 〈0.01）, but neither of them attainef the levels in NS group. Conclusion GABAA receptor bears a part in the impairment of memory by ketannne in mice.
出处
《徐州医学院学报》
CAS
2006年第3期189-191,共3页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学基金(39970715)资助项目
江苏省自然科学基金(BK2001143)资助项目
