摘要
为探讨哀老大鼠脑 M 受体基因表达的变化和滋阴药知母及其有效成份 ZMS 的作用,用原位杂交技术和图像分析仪进行了 m1和 m2受体 mRNA 的定量分布研究。结果:老年大鼠脑纹状体部位的 m1 mRNA 明显减少(灰度值降低12.26±3.60);长期口服 ZMS 的老年大鼠,该部位 m1 mRNA 显著高于对照老年鼠(灰度值提高15.71±3.27),长期口服知母者平均灰度值也略有提高。老年大鼠大脑皮层和海马部位未见 m1 mRNA 显著降低,也未见 ZMS 或知母有明显作用。m2 mRNA 在以上一些部位未见特异性灰度改变,可能与这些部位 m2 mRNA 含量不丰有关。因此,ZMS 对老年大鼠脑纹状体部位 m1 mRNA 的明显减少有改善作用。
PURPOSE To study the change of gene expression of m1 receptors in different brain areas of aged rats and the effects of water extract of the Yin tonic Zhimu and its active principle ZMS.METHODS In situ hybridization using ^(35)S-labelled m1 and m2 probes and analysis of the autoradiographs using a computerized image-analyzer.RESULTS The grain density of m1 mRNA in striatum was significantly lowered in old rats as compared with young rats (decreased by 12.26±3.60,P<0.01). Long-term oral administration of ZMS,the active principle of Yin tonic Zhimu but not the water extraction of Zhimu,elevated the m1 mRNA in striatum of aged rats (increased by 15.71±3.27,P<0.01).Neither significant change of the grain density of m1 mRNA in old rats nor significant effect of Zhimu or ZMS was observed in cerebral cortex and hippocampus.CONCLUSIONS The m1 mRNA level in striatum is decreased in aged rats and ZMS is able to elevate it.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1996年第3期152-154,共3页
Chinese Journal of Nuclear Medicine
基金
国家自然科学基金
上海市高教局科学发展基金资助
关键词
胆碱能
知母
原位
MRNA
衰老
Receptors
cholinegic
Brain
RNA
messenger
Anemarrkena asphodeloides unge HybridizationRats
