摘要
目的:检测湛江地区初次献血者丙型肝炎病毒(HCV)-RNA,调查初次献血人群HCV感染的“窗口期”情况以及感染HCV的具体基因型。方法:分别采用ELISA法和RT-PCR法检测2 357 名在2004年1-12月初次无偿献血者感染HCV的情况。并把经RT-PCR法检测阳性的标本进行基因型检测。结果:在2 357名初次献血者中,ELISA法阳性率为1．64％,而RT-PCR法的阳性率为 1．86％,2种方法符合率为99．5％,二者间无显著性差异(P>0．05)。感染者中2型基因型为84．1％,3 型基因型为13．6％,2／3混合型为2．27％。结论:RT-PCR法检测血液HCV-RNA能早期发现“窗口期”HCV感染者,湛江地区初次无偿献血者感染丙型肝炎病毒的基因型以2型和3型为主。
Objective： To examine the Hepatitis C （HCV）infection status of first-time blood donors in Zhanjiang city and to investigate both early stage of HCV infection and its HCV gene types in the blood donors for the first time in Zhanjiang. Methods： The total 2 357 donors＇ samples from January to December in 2004 were tested by ELISA and RT-PCR technique respectively. The positive samples were collected for testing HCV gene types again. Results： 1.64% and 1.86% of 2 357 blood donors were positive by ELISA and RT-PCR respectively. There were no significant dit^erences between ELISA and RT-PCR methods（P〉0.05）. HCV-2,HCV-3 and HCV-2/3 mixed type were 84.1%, 13.6%,2.27% in the infectors respectively. Conclusions： RT-PCR method can identify HCV infected donors at an early stage and HCV-2 and HCV-3 are predominant HCV gene type in Zhanjiang city.
出处
《海南医学院学报》
CAS
2006年第2期115-117,共3页
Journal of Hainan Medical University
关键词
聚合酶链反应
供血者
湛江市
丙型肝炎
Hepatitis virus group, C type
PCR
Blood donors
Zhanjiang city
