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二乙基亚硝胺诱导转化的肝癌肝组织小鼠中SP细胞的观察 被引量：4

Observation of side population cells in mice liver tumor induced by diethylnitrosamine
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摘要 目的:研究小鼠肝癌组织中的SP细胞,从而推测肝癌干细胞(CSC)存在的可能性。方法:首先用致癌剂二乙基亚硝胺(DEN)诱导B6C3F1雄性小鼠发生肝癌,在无菌条件下,将肝癌组织消化成单细胞悬液,然后用荧光染料Hoechst 33342对细胞悬液进行染色分析。结果:在原代分离的小鼠肝癌细胞群中观察到SP细胞的存在。结论:实验结果推测,在药物诱导转化的小鼠肝癌组织中很可能存在CSC。 Objective ：The tumor, and to speculate the step is to induce liver tumor aims of this experiment is to find side population （SP） cells in mouse liver existence of liver tumor stem cell. Methods：In this experiment, the first in male B6C3F1 mice by carcinogen diethylnitrosamine and obtain the singlely suspened cells at sterile conditions. Then the SP phenotype of these singlely suspened cells were diplayed by dyeing with fluorescent Hoechst 33342. Results：The SP cells were observed in the singlely suspended primary cells of mouse liver tumors. Conclution ： The results of this experiment indicated the existence of liver cancer stem cells.
作者 张朵 朱海英 谢东甫 王新民 李建秀 胡以平
机构地区 第二军医大学基础医学部细胞生物学教研室
出处 《医学研究生学报》 CAS 2006年第5期424-427,i0010,共5页 Journal of Medical Postgraduates
基金 国家自然科学基金资助项目(批准号:30470876)
关键词 转化 SP细胞 肝癌 二乙基亚硝胺 Transform Side population cell Cancer stem cell
分类号 R735.7 [医药卫生—肿瘤]
  • 相关文献

参考文献18

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共引文献10

同被引文献24

  • 1陈凌华,程祖锌,杨志坚,黄志伟,许明.灵芝多糖热水提取条件的优化试验[J].浙江食用菌,2009(4):21-23. 被引量：16
  • 2王红晖,聂捷琳,李莹辉,戴钟铨.成体干细胞中的SP细胞群[J].中国生物工程杂志,2005,25(12):75-77. 被引量：1
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引证文献4

二级引证文献6

医学研究生学报
2006年 第5期

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